丙酮酸乙酯对脑出血大鼠血清肿瘤坏死因子α和白介素6水平的影响

Influence of Ethyl Pyruvate on Serum Levels of TNF-α and IL-6 in Rats with Cerebral Hemorrhage

目的探讨丙酮酸乙酯对脑出血大鼠血清肿瘤坏死因子α(TNF-α)、白介素6(IL-6)水平的影响。方法采用自体动脉血制备脑出血模型,将48只清洁级SD大鼠随机分为假手术组(F组,n=8)、脑出血组(ICH组,n=20)和丙酮酸乙酯干预组(EP组,n=20)。F组大鼠只进针,不注血;ICH组大鼠建立脑出血模型后腹腔注射0.9%氯化钠溶液;EP组大鼠建立脑出血模型后腹腔注射丙酮酸乙酯,每组大鼠按建模时间(6 h、24 h、3 d、7 d)不同分为4个亚组。评估各组大鼠不同建模时间点神经功能缺损程度,采用酶联免疫吸附试验(ELISA)法检测大鼠不同建模时间点血清TNF-α、IL-6水平。结果 ICH组不同建模时间点神经功能缺损评分低于EP组,EP组低于F组(P<0.05)。ICH组大鼠不同建模时间点血清TNF-α水平高于F组和EP组,ICH组和EP组大鼠不同建模时间点血清IL-6水平高于F组,除建模24 h外ICH组大鼠血清IL-6水平均高于EP组(P<0.05)。结论脑出血后大鼠神经功能受损,血清TNF-α、IL-6等炎性因子水平升高。丙酮酸乙酯能降低血清TNF-α、IL-6等炎性因子水平,抑制炎性反应,对脑出血大鼠具有脑保护作用。

Objective To investigate the influence of ethyl pyruvate on serum levels of TNF-α and IL-6 in rats with cerebral hemorrhage. Methods A total of 48 specific pathogen free Sprague-Dawley rats were selected and randomly divided into groups A（ n = 8）,B（ n = 20）,C（ n = 20）. A group was given sham-operation,B group was given autologous arterial blood to build cerebral hemorrhage model and then given intraperitoneal injection of 0. 9% sodium chloride solution,C group was given autologous arterial blood to build cerebral hemorrhage model and then was given intraperitoneal injection of ethyl pyruvate. After 6 hours,24 hours,3 days,7 days of intervention,2,5,5 rats were randomly picked out from groups A,B,C,respectively,and the degree of nervous functional defects was evaluated,ELISA to used to detect the serum levels of TNF-α and IL-6. Results The nervous functional defects score of B group was lower than that of C group at each time point,respectively,and that of C group was lower than that of A group,respectively（ P〈0. 05）. The serum TNF-α level of B group was higher than that of groups A and C at each time point,respectively; the serum IL-6 level of groups B and C was higher than that of A group at each point,respectively,and serum IL-6 level of B group was higher than that of C group after 6 hours,3days,7 days of intervention. Conclusion Rats with cerebral hemorrhage have different degrees of nervous functional defects,and the serum levels of TNF-α and IL-6 are higher; ethyl pyruvate can reduce the serum levels of TNF-α and IL-6,inhibit the inflammatory reactions,has some cerebral protection effect.