亚洲玉米螟Pgi基因全长的同源PCR克隆

Homologous PCR Cloning of Whole Pgi Gene in Ostrinia furnacalis

为从核酸水平上研究亚洲玉米螟 Pgi 基因，以 GenBank 中的鳞翅目昆虫 Pgi 基因 mRNA 序列为参考序列，利用 MEGA 5．0软件、IDT 在线程序及 Primer Primer5．0软件等设计用于扩增亚洲玉米螟Pgi 基因编码序列的引物，同时利用 PCR 扩增及序列分析验证引物的有效性。结果表明：测定的亚洲玉米螟 Pgi 基因编码序列与 Genbank 中美国白蛾、苜蓿黄蝶等鳞翅目昆虫 Pgi 基因 mRNA 序列均具有较高的相似度（达78％～85％），表明，设计的引物均具有较强的有效性，能成功地扩增出亚洲玉米螟 Pgi 基因编码序列。

The coding sequence of O.furnacalis Pgi gene was amplified by the primer designed by MEGA 5.0 software,IDT on-line procedure and Primer Primer5.0 software and by taking Lepidopteron Pgi gene mRNA sequence in Genbank as a reference sequence,and the primer’s effectiveness was tested by PCR amplification and sequence analysis to study O.fiurnacalis Pgi gene at the nucleic acid level.The results showed that the similarity degree between the coding sequence of O.furnacalis Pgi gene and the Pgi gene mRNA sequence of Hyphantria cunea and Colias eurytheme in Genbank is up to 78%～85%, which indicates that the designed primer is of higher effectiveness and can be successfully used in amplification of O.furnacalis Pgi gene coding sequence.