摘要
为了提高桃树的耐淹水能力,以毛桃为材料,淹水胁迫12d后观测丛枝菌根真菌(AM真菌)摩西球囊霉(Funneliformis mosseae)对其植株鲜重、菌根侵染率、根系脯氨酸含量、脯氨酸合成酶(△1-吡咯啉-5-羧酸合成酶和鸟氨酸转氨酶)、脯氨酸分解酶(脯氨酸脱氢酶)的影响。结果表明:淹水处理显著降低了F.mosseae对根系的侵染;在非淹水和淹水处理下,接种F.mosseae真菌显著增加了植株鲜重、根系的脯氨酸含量和脯氨酸合成酶活性,但显著降低了鸟氨酸转氨酶和脯氨酸脱氢酶活性。因此,推测AM真菌主要通过提高谷氨酸合成途径和降低脯氨酸的分解,从而促进根系脯氨酸的积累,提高桃的耐淹性。
The effect of F.mosseae (AM fungi)on plant fresh weight,mycorrhiza infection rate, proline content in roots,activity of △ 1-pyrroline-5-carboxylate synthase (P5CS),orn-δ-aminotransferase (δ-OAT)and proline dehydrogenase (ProDH)of wild peach under 12d waterlogging stress was analyzed to improve waterlogging tolerance of peach.The results showed that waterlogging treatment reduces F. mosseae infection rate of roots significantly and under non-waterlogging and waterlogging stress, inoculating AM fungi can significantly increase plant fresh weight,proline content of roots and activity of P5CS,but significantly reduce activity ofδ-OAT and ProDH.Therefore,AM fungi may promote proline accumulation in roots of peach to enhance its waterlogging tolerance by improving synthetic path of glutamic acid and reducing proline catabolism.
出处
《贵州农业科学》
CAS
2015年第3期51-53,57,共4页
Guizhou Agricultural Sciences
基金
长江大学大学生创新创业训练计划项目"丛枝菌根真菌改变桃耐涝性的脯氨酸机制"(104892013042)
关键词
淹水胁迫
菌根
脯氨酸
脯氨酸合成酶
waterlogging stress
mycorrhiza
proline
proline synthetase