119例疑似非输血感染者抗-HCV与HCVRNA检测结果比较与分析

Analysis and Comparison of anti-HCV and HCV RNA results of 119 patients with suspicious no-transfusion infection

目的对119例疑似医源性非输血感染丙肝患者的血液进行HCV检测。方法采用2种第3代酶免抗-HCV试剂检测,并同时进行单人份核酸HCV RNA检测。结果 119例疑似HCV血液用万泰酶免试剂检出抗-HCV(+)68例,阳性率为57.14%;丽珠酶免试剂检出抗-HCV(+)61例,阳性率为51.26%。酶免双试剂检出抗-HCV(+)61例,反应符合率为89.70%。万泰酶免抗-HCV(+)最小S/CO值是0.79,最大S/CO值是21.43,平均S/CO值是7.95,中位数是6.23;丽珠酶免抗-HCV(+)最小S/CO值是0.66,最大S/CO值是17.65,平均S/CO值是4.23,中位数是2.87。浩源核酸试剂检出HCV RNA(+)98例,阳性率为82.35%。万泰酶免抗-HCV(+)和浩源核酸HCV RNA(+)63例,丽珠酶免抗-HCV(+)和浩源核酸HCV RNA(+)56例。万泰和丽珠酶免试剂检出灰区的样本,浩源核酸试剂检测全部是阳性。5例酶免双试剂抗-HCV(+),核酸是HCV RNA(-)。结论酶免和核酸方法检测HCV结果存在差异,联合应用酶免和核酸方法筛查血液传染性疾病更能保证输血安全。

Objective Detect anti-HCV in 119 hepatitis C patients serum with suspicious iatrogenic non-transfusion. Methods The anti-HCV was monitored by two kinds of enzyme-linked immunosorbent assay reagents in the third generation and serum levels of HCV RNA quantities were detected by real-time PCR. Results 68 anti-HCV positive samples were detected by Wantai ELA reagent in 119 cases,the positive rate was 57. 14%; While 61 were tested by Lizhu ELA reagent,the positive rate was 51. 26%. 61 positive cases were detected with dual reagent,reactive coincidence rate was 89. 70%. In Wantai reagent anti-HCV positive cases,the minimum S / CO was 0. 79,maximum S / CO was 21. 43,average S / CO was 7. 95,median was6. 23; While Lizhu reagent,the S / CO was 0. 66,17. 65,4. 23,2. 87,respectively. 98 positive HCV RNA were measured in Haoyuan PCR system,positive rate was 82. 35%; Among which,63 and 56 were detected respectively at anti-HCV and HCV RNA level by Wantai-Haoyuan and Lizhu-Haoyuan reagent,samples in the gray zone detected by both Wantai and Lizhu were positive in Haoyuan system,5 samples were reactive by dual reagent while HCV RNA level were negative. Conclusion There are differences enzyme immunoassay and nucleic acid method in the detection of HCV,to combined of ELISA and nucleic acid method and applied to screen blood borne diseases and ensure the safety of blood transfusion.