结直肠腺癌芳香烃受体核转运蛋白的表达

Expression and significance of aryl hydrocarbon receptor nuclear translocator in colorectal adenocarcinoma

目的 探讨芳香烃受体核转运蛋白（ARNT）在结直肠腺癌和正常大肠黏膜中的表达及临床病理特征.方法 分别应用实时荧光定量聚合酶链反应（FQ-PCR）、Western blot和免疫组织化学技术检测44例结直肠腺癌患者的手术标本（肿瘤组织、大肠组织）中ARNT的表达情况,并结合性别、年龄、肿瘤病理特征等进行相关性分析.结果 与正常大肠组织比较,结直肠腺癌组织中ARNT mRNA（33/44）和蛋白水平（28/44）呈低表达状态.免疫组织化学检测结果显示大肠黏膜ARNT高表达37例（84.1％）远高于肿瘤组织的22例（50.0％）,相关性分析提示ARNT高表达与肿瘤分化呈正相关、TNM分期呈负相关.结论 ARNT基因在结直肠腺癌组织为低表达状态.

Objective To investigate the expression level and the clinical pathological significance of aryl hydrocarbon receptor nuclear translocator （ARNT） in colorectal adenocarcinoma.Methods 44 pairs of tumor and adjacent non-cancerous tissues were collected form radical resection of colorectal carcinoma.Real-time fluorescent quantitative polymerase chain reaction （FQ-PCR）,Western blotting and immunohistochemistry were used to dectect the expression of the ARNT.Analysis the correlation between the expression and the clinical pathology parameters.Results The expression of ARNT in adenocarcinoma were significantly lower than the adjacent non-cancerous tissues both in mRNA （33/44） and protein （28/44）.ARNT high expression were 22/44 （50.0%） in tumors while 37/44 （84.1%） in adjacent non-cancerous tissues.The expression of ARNT was correlated with the TNM stages and tumor differentiation.Conclusion ARNT indicates as a cancer suppressor gene and its expression was decreased in colorectal adenocarcinoma.