蛋白酪氨酸磷酸酶-3在肿瘤微环境中对结肠癌LoVo细胞Snail表达的调控

Phosphatase of regenerating liver-3 regulates the expression of Snail in human colon cancer cells LoVo through tumor microenvironment

目的 观察结肠癌肝转移的肿瘤微环境中肿瘤细胞与肝细胞（L02）相互作用及对肿瘤细胞Snail基因表达的影响.方法 将转入蛋白酪氨酸磷酸酶-3（PRL-3）的LoVo细胞和人正常肝细胞（L02）模拟肿瘤微环境进行共培养0～5d后,通过反转录-聚合酶链反应（RT-PCR）和Western blot检测LoVo细胞Snail蛋白表达,通过细胞划痕实验检测LoVo细胞迁移能力的改变.结果 用RT-PCR和Western blot检测发现PRL-3能通过共培养降低Snail基因的表达,蛋白灰度分析显示其抑制率分别为34.06％、37.82％、39.91％、64.07％、73.58％ （P＜0.01）,而未转染的LoVo细胞Snail表达降低不明显,并且经过共培养后高表达PRL-3的LoVo细胞迁移能力明显降低（P＜0.05）.结论 PRL-3在与肝细胞的共培养环境中下调肿瘤细胞Snail表达从而降低LoVo细胞的迁移能力.

Objective To investigate the interaction between colon cancer cells and hepatic cell （L02）,and it＆#39; s influence to Snail expression in tumor cells.Methods LoVo cells which transfected with phosphatase of regenerating liver-3 （PRL-3） and human hepatic cells （L02） simulated tumor microenvironment were cocultured by 0-5 day,reverse transcriptase-polymerase chain reaction （RT-PCR） and Western blotting were used to examine Snail expression of LoVo cells,and the migration of LoVo cells also be detected.Results Used by RT-PCR and Western blotting it was found out that LoVo cells （transfected with PRL-3） were reduced Snail express after cocultured with hepatic cells,the inhibition rates were 34.06%,37.82%,39.91%,64.07%,73.58% （P 〈 0.05）,LoVo cells （without transfected PRL-3） decreased expression of Snail was not obvious.Besides,coculture also depress the migration of LoVo cells.Conclusion PRL-3 depress the migration of LoVo cells through reduced Snail expression in liver metastasis of colon cancer microenvironment.