核受体辅激活蛋白5在结直肠癌中的表达及其临床意义

The expression and clinical significance of nuclear receptor co-activator 5 in colorectal cancer

目的 探讨核受体辅激活蛋白5（NCOA5） mRNA及蛋白在结直肠癌组织中的表达及其与临床病理特征的关系.方法 采用实时荧光定量聚合酶链反应（FQ-PCR）和Western blot法检测74例结直肠癌患者癌组织中NCOA5mRNA和蛋白表达水平,并与临床病理特征进行统计学分析.结果 结直肠癌患者的癌组织中NCOA5 mRNA （74.3％）和蛋白（83.8％）的表达水平低于癌旁组织.结直肠癌组织中NCOA5mRNA和蛋白的表达与性别、年龄、肿瘤大小、肿瘤Dukes分期及淋巴结转移无明显相关,而与肝脏转移和分化程度显著相关（P＜0.05）.结论 NCOA5的低表达可能参与结直肠癌的发生和转移.

Objective To investigate the expression of nuclear receptor co-activator 5 （NCOA5） in colorectal cancer tissues,and its correlation with clinicopathologial features.Methods The expression levels of NCOA5 mRNA and protein in 74 cases of colorectal cancer tissues were separately detected by real-time fluorescent quantitative polymerase chain reaction（FQ-PCR） and Western blotting.Meanwhile,we statistically analyzed the relationship between its expression levels and clinical data.Results The positive expression rate of NCOA5 mRNA （74.3%） and protein （83.8%） in colorectal cancer tissues was significantly lower than in the adjacent tissues.The expression of NCOA5 mRNA and protein in colorectal cancer tissues was significantly correlated with liver metastasis and differentiation （P 〈 0.05）,but not with gender,age,tumor size,Dukes stage and lymph node metastasis of colorectal cancer.Conclusion The low expression of NCOA5 may promote the development and metastasis of colorectal cancer,and can be a therapeutic target in patients with colorectal cancer.