Girdin蛋白在大肠癌组织中的表达及其与侵袭转移的关系

Expression of Girdin protein and its correlation with tumor＆#39;s invasion and metastasis in colorectal carcinoma

目的 检测大肠癌组织中Girdin蛋白的表达,探讨与大肠癌临床病理特征及侵袭转移的关系.方法 应用免疫组织化学Envision二步法,对84例大肠癌及40例腺瘤、30例癌旁黏膜组织进行Girdin蛋白检测,统计分析其与临床病理参数之间的关系.结果 84例大肠癌组织中Girdin蛋白阳性表达率为42.9％,癌旁黏膜组织、低级别上皮内瘤变（LGIN）腺瘤及高级别上皮内瘤变（HGIN）腺瘤Girdin蛋白阳性表达率分别为3.3％、11.1％、18.2％,差异有统计学意义（x2=15.752,P＜0.01;x2=6.391,P＜0.05x2 =4.518,P＜0.05）.大肠癌组织中Girdin蛋白的表达与肿瘤浸润深度、淋巴结转移及临床分期明显相关（P＜0.01）,与性别、年龄、肿瘤大小、肿瘤部位、组织学分级无明显相关（P＞0.05）.大肠癌Girdin蛋白阳性表达组的5年生存率为30.6％,阴性组5年生存率为83.3％,两组间差异有统计学意义（P＜0.01）.结论 Girdin蛋白在大肠癌组织中呈高表达,与大肠癌的侵袭转移有关.

Objective To investigate the expression of Girdin protein in colorectal carcinoma,and explore its relationship with clinical characteristics and tumor＆#39; s invasion and metastasis.Methods Eighty-four cases of colorectal carcinoma specimens,40 cases of colorectal adenoma and 30 cases of tumor-adjacent mucosal tissues were collected,and the expression levels of Girdin protein were detected by immumihistochemistry Envision two-step methods.The relationship between Girdin expression and clinicopathological characteristics was analyzed.Results The positive expression rate of Girdin protein in colorectal carcinoma was 42.9%,which was significantly higher than that in tumor-adjacent mucosa,adenoma with low grade inraepithelial neoplasia （LGIN） and adenoma with high grade inraepithelial neoplasia （HGIN） respectively （x2 =15.752,P 〈 0.01 ;x2 =6.391,P 〈 0.05 ;x2 =4.518,P 〈 0.05）.Overexpression of Girdin protein was correlated with invasion depth,lymph node metastasis and Dukes clinical stage （P 〈 0.01）,but not with gender,age,pathologic grade,tumor＆#39;s size and localization （P 〉 0.05）.Follow-up study results showed that the five-year overall survival rate in Girdin negative group （83.3%） was higher than that in Twist positive group （21.7%） significantly （P 〈 0.01）.Conclusion Girdin protein expression is up-regulated in colorectal carcinoma and correlated with tumor＆#39;s invasion and metastasis.