丙泊酚对肺癌A549细胞凋亡的影响及其机制

Influence of propofol on proliferation and apoptosis of human lung cancer A549 cells

目的 探讨丙泊酚对肺癌A549细胞凋亡的影响及其机制.方法 用噻唑蓝（MTT）法检测丙泊酚对肺癌A549细胞增殖作用的影响,采用流式细胞技术检测丙泊酚对A549细胞细胞凋亡的影响,Western blot测定丙泊酚对A549细胞水通道蛋白1（AQP1）表达的影响,应用Transwell小室实验检测AQP1蛋白表达对肺癌A549细胞迁移的影响.结果 MTT法和流式细胞法显示不同浓度丙泊酚作用A549细胞株48h后,细胞抑制率和凋亡率分别为（52.8±5.2）％、（61.2±5.9）％、（69.4±6.1）％;（42.8±4.1）％、（59.8±11.2）％、（71.4±13.4）％.Western blot结果显示不同浓度丙泊酚作用A549细胞48h后,AQP1蛋白的表达水平为0.87±0.12、0.54±0.07、0.31 ±0.04;ranswell小室实验显示不同浓度丙泊酚作用于A549细胞后,迁移细胞数分别为（61.35±8.98）、（51.78±7.58）、（40.35±5.12）个.结论 丙泊酚对人肺癌A549细胞的生长有明显的抑制作用,诱导肿瘤细胞凋亡,并能抑制肺腺癌细胞的迁移能力,其机制可能与下调AQP1蛋白表达有关.

Objective To investigate the influence of propofol on lung cancer cell proliferation and apoptosisin vitro.Methods MTT assay was used to detect the inhibition effect of propofol on the growth of A549.Flow cytometry was applied to detect the impact of propofol on apoptosis of A549 cells.Western blotting was used to determine the effect of propofol on aquaporin 1 （AQP1） protein expression in A549 cells,and the migration ability of A549 cells was investigated by Transwell chamber.Results MTT assay and flow cytometry showed that the rate of inhibition and apoptosis in the experiment group after propofol actingon A549 were （52.8±5.2）%,（61.2 ±5.9）%,（69.4 ±6.1）% ; （42.8 ±4.1）%,（59.8 ± 11.2）%,（71.4 ± 13.4）%,respectively.The expression of AQP1 protein was 0.87 ± 0.12、0.54 ± 0.07、0.31 ± 0.04; and the migration ability of A549 cells was 1.35 ± 8.98、51.78 ± 7.58、40.35 ± 5.12.Conclusion Propofol can significantly inhibit the proliferation,apoptosis and migration ability of human lung cancer A549 cells,which may be associated with the down-regulation of AQP1 protein.