扶正解毒散及其超微粉体外抗鸡传染性法氏囊病病毒药效比较

Effects Comparison of Fuzheng Jiedu San and its Super Micropowder on Anti-IBDVin Vitro

采用鸡胚法和细胞法2种体外抗病毒试验比较扶正解毒超微粉(FZSM)和扶正解毒散(FZ)的抗鸡传染性法氏囊病病毒(IBDV)效果。鸡胚法:在FZSM和FZ 2种煎煮液对鸡胚安全浓度范围内分别选取3个浓度,采用先加煎煮液后接种病毒和先接种病毒后加煎煮液2种方式,接种10日龄SPF鸡胚,测定2种煎煮液对IBDV ELD50的影响。细胞法:在FZSM和FZ 2种煎煮液对鸡胚成纤维细胞(CEF)安全浓度范围内分别选取3个浓度,采用先加煎煮液后接种病毒和先接种病毒后加煎煮液2种方式,加入到长成单层的CEF中继续培养,观察并统计各孔的细胞病变(CPE)。结果显示,FZSM和FZ 2种煎煮液均可以降低IBDV的病毒滴度和CPE抑制率,高浓度效果优于低浓度;同浓度FZSM效果优于FZ;预防作用效果优于治疗作用效果。结果表明FZSM和FZ均具有体外抗IBDV的作用,且FZSM效果优于FZ。

In this study,we compared the effects of Fuzheng Jiedu super micropowder （FZSM） and Fuzheng Jiedu san （FZ） on anti-IBDV in vitro. Method of chicken embryo, 10-day-old SPF chicken embryos were inoculated with FZSM and FZ in three safe concentrations by two pathways of adding decoction before inoculation of virus and adding decoction after inoculation of virus, and then the effects of two decoctions on ELDs0 of IBDV to 10-day-old SPF chicken embryos were measured. Method of cell,CEF was inoculated with FZSM and FZ in three safe concentrations by two pathways of adding decoction before inoculation of virus and adding decoction after inoculation of virus,and then the effects of two decoctions on CPE of IBDV to CEF were observed. The results showed that the virus titer and inhibition rate of CPE of IBDV were reduced by FZSM and FZ,the effect of high concentration was better than its low concentration, at the same concentration,the anti-IBDV effect of FZSM was superior to that of FZ,and preventive effect was better than therapeutic action. These results indicated that FZSM and FZ had the function of anti-IBDV in vitro and the effect of FZSM was superior to FZ.