α-常春藤皂苷抗肿瘤作用机制研究

Anti-tumor Effect and Mechanisms of Alpha-Hederin

目的探讨α-常春藤皂苷(BD)对人脑胶质母细胞瘤细胞U251抗肿瘤的作用机制。方法MTT法、集落形成、吉姆萨染色检测不同浓度BD对U251细胞增殖影响;Hochest33342观察细胞凋亡形态变化;流式细胞术检测细胞凋亡;Western Blot检测Bcl-2、Caspase-3蛋白凋亡变化。结果BD可以明显抑制U251的增殖,其抑制作用呈明显的时间、剂量依赖关系。在给药24h后,呈强蓝色荧光,并且细胞在BD18.75,25.00μmol·L-1出现显著凋亡,下调Bcl-2,Caspase-3蛋白表达。结论BD能显著地抑制U251细胞的增殖、诱导细胞凋亡,其作用机制可能与线粒体凋亡有关。

Objective To study the anti-tumor effect and mechanism of alpha-hederin（BD） on human brain glioblastoma cell line U251.Methods MTT assay,colony formation test,and Giemsa staining method were used for observing the effect of different concentrations of BD on the proliferation of U251 cells.Hochst33342 assay kits were adopted to observe the morphology of apoptotic cells.Flow cytometry was used to detect the apoptosis of tumor cells,and the expression levels of Caspase-3 and Bcl-2 protein were evaluated by Western blot assay.Results BD exhibited an inhibitory effect on U251 cell growth in concentration-dependent and time-dependent manner.After treatment for 24h,a strong blue fluorescence was presented in U251 cells.BD at the concentrations of18.75,25.00μmol·L-1 could induce significant apoptosis and down-regulate the expression levels of Bcl-2 and Caspase-3.Conclusion BD could inhibit the proliferation of U251 cells and induce apoptosis,and the mechanism may be related to mitochondrial apoptosis pathway.