摘要
目的：探讨人参皂甙肠道代谢物Compound K（CK）对人肝癌细胞MHCC97-H增殖及侵袭的影响，并初步探讨及可能的机制。方法噻唑蓝（MTT）比色法检测CK对细胞增殖活性的影响；流式细胞仪检测CK对MHCC97-H细胞周期的影响；Transwell小室法检测细胞侵袭能力；逆转录-聚合酶链反应及Western blot法分别检测p21、MMP-2及MMP-9 mRNA及蛋白的表达水平。结果与对照组相比，CK处理组的细胞增殖活性明显下降，细胞周期分析显示，随着CK浓度的增加，G0/G1期细胞所占百分率逐渐增加；CK作用肝癌细胞48 h后，侵袭穿膜细胞数分别为（22.24±7.3）、（18.24±4.6）个，与对照组比较差异有统计学意义（P〈0.01）；RT-PCR及western blot结果显示CK能够促进p21的表达，下调MMP-2及MMP-9的表达。结论CK能够显著抑制人肝癌细胞的增殖和侵袭，机制可能与阻滞细胞周期以及影响相关基因表达有关。
ObjectiveTo investigate the effects of CK on proliferation and invasion of human liver cancer MHCC97-H cells, and appraise the possible mechanism.MethodsWith the treatment of CK in vitro, cell viability was assayed using MTT methods; Cell invasion of MHCC97-H cells were assessed by transwell technique. and the p21 and MMP-2/9 were detected by real-time RT-PCR and western-blot respectively.ResultsCompared with the control,CK decreased the proliferation of MHCC97-H cells, The colony formation ability of MHCC97-H cells was decreased and percentage of cells in G0/G1 phase was increased in a dose-dependent manner, as compared to control. The cell invasion abilities were decreased gradually. The real-time RT-PCR showed that p21 mRNA expression was increased and the MMP-2/9 mRNA was decreased.ConclusionCK inhibits the proliferation and invasion of MHCC97-H cells mainly by blocking the cell cycle at G0/G1 and down-regulating the expression of related gene factors.
出处
《中国临床实用医学》
2015年第1期17-20,共4页
China Clinical Practical Medicine
基金
滨州医学院科技发展项目（BY2013KJ91）
