324例RhD(—)孕妇抗D抗体检测及分析

Detection and analysis of anti-D antibody in RhD(—)pregnant

目的:检测和分析RhD(-)孕妇外周血中抗D抗体效价,评价新生儿溶血病(HDN)的发生率。方法:收集北京积水潭医院和北京大学人民医院2012-2013年的RhD(-)孕妇外周血,采用血清学方法对所有标本进行Rh血型鉴定,谱细胞进行抗体检测和鉴定,并检测抗D抗体效价。结果:孕妇外周血中抗D抗体的检出率为8.6%,有既往妊娠史的孕妇抗D抗体的检出率明显高于第一次妊娠的孕妇。结论:产前检测和分析RhD(-)孕妇体内的抗D抗体,可以预测HDN发生的概率。

Objective：To detect and analyze the anti-D titer in the peripheral blood of RhD（-）pregnant,and evaluate the incidence of hemolytic disease of newborn（HDN）.Method：The peripheral blood of RhD（-）pregnant was collected from hospitals.We used serological test to detect Rh blood group,panel cell to detect and identify the anti-D antibody,and detect the titer of antibody furtherly.Result：The detection rate of anti-D antibody in the peripheral blood pregnant was 8.6%,and it was significantly higher in pregnant with previous pregnancy history than that in the pregnant in first pregnancy.Conclusion：Prenatal detection and analysis of anti-D antibody in RhD（-）pregnants could predict the occurrence of HDN.