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针刺对应激性胃黏膜损伤大鼠睡眠时间及纹状体中五羟色胺、多巴胺含量的影响 被引量:17

Effects of Acupuncture on Sleeping Time and Striatum 5-HT and DA Content in Rats with Stress Induced Gastric Mucosal Injury
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摘要 目的:从脑-肠轴角度探讨针刺不同穴位对大鼠应激性胃黏膜损伤的愈合及睡眠情况的影响。方法将雄性Wistar大鼠随机分为空白组、模型组、治胃组、安神组、联合组,每组8只。采用无水乙醇灌胃方法造成应激性胃黏膜损伤大鼠模型,治胃组穴选双侧“足三里”“中脘”,安神组穴选双侧“申脉”“照海”,联合组穴选双侧“足三里”“中脘”“申脉”“照海”,用0.22 mmx13 mm不锈钢毫针刺入5-10 mm,留针20 min。针刺干预5 d后检测热痛阈的变化。按40 mg/kg大鼠体质量腹腔注射2%戊巴比妥钠,记录注射后大鼠的睡眠持续时间。肉眼观察大鼠的胃黏膜形态,Guth法计算黏膜损伤指数(UI),高效液相色谱-电化学检测法测纹状体中5-羟色胺(5-HT)、多巴胺(DA)的含量。结果与空白组比较,模型组热痛阈和睡眠持续时间显著下降(P<0.01),UI 及5-HT、DA 含量显著升高(P<0.05,P<0.01);与模型组比较,各治疗组热痛阈和睡眠持续时间增加(P<0.05,P<0.01),UI均显著降低(P<0.01),治胃组和联合组中5-HT含量明显降低、治胃组DA含量显著降低(P<0.01);各治疗组间比较,联合组热痛阈及睡眠持续时间显著增加(P<0.01),UI显著降低(P<0.05)。结论针刺足三里、中脘、照海、申脉可促进大鼠胃黏膜损伤的愈合,同时延长睡眠持续时间,其中四穴联合应用效果更佳,其机制可能是通过调节纹状体中5-HT及DA含量发挥作用。 Objective To observe the effects of acupuncture on the sleeping quality of male Wistar rats with stress induced gastric mucosal injury from the viewpoint of the brain-intestine axis. Methods Male Wistar rats were randomized into normal control, model, ST36-CV12, BL62-KI6, and combined groups, with 8 rats in each group. The model was established by using intragastric perfusion of ethanol in the rats. Acupoints of Zusanli (ST36) and Zhongwan (CV12) on both sides were used for the ST36-CV12 group. Acupoints of Shenmai (BL62) and Zhaohai (KI6) on both sides were used for the BL62-KI6 group. All four acupoints were used for the combined group. 0.22 mm x13 mm stainless steel filiform needles were inserted 5-10 mm deep, and were left untouched for 20 minutes before withdrawal. The acupoints were stimulated each day for five days and the paw withdraw thermal latency (PWTL) was detected after the last stimulation. 2%pentobarbital sodium was then injected (40 mg/kg), and sleeping time of rats was detected. Gastric mucosa was observed through naked eyes. Gastric mucosal ulcer Index was determined by using the Guth method. 5-HT and DA contents were detected by using HPLC-ECD method. Results Compared with the normal control group, PWTL and sleeping time decreased (P〈0.01), and UI, 5-HT, and DA content increased in model group (P〈0.05, P〈0.01). Compared with the model group, PTWL and sleeping time increased (P〈0.05, P〈0.01), and UI decreased in all treatment groups (P〈0.01). 5-HT content in ST36-CV12 and combined groups decreased significantly, and DA content in ST36-CV12 group dropped (P〈0.01). Compared with other treatment groups, PWTL and sleeping time increased significantly (P〈0.01), and UI decreased obviously in combined group (P〈0.01). Conclusion Stimulating acupoints of Zusanli, Zhongwan, Shenmai, and Zhaohai (KI6) may promote the recovery of gastric mucosal injury while at the same time increase sleeping time, with four acupoints used in combination having the best efficacy. The mechanism may be related to adjusting contents of 5-HT and DA in corpus striatum.
出处 《中国中医药信息杂志》 CAS CSCD 2015年第5期48-51,共4页 Chinese Journal of Information on Traditional Chinese Medicine
基金 北京中医药大学自主选题项目(53210100604216)
关键词 应激性胃黏膜损伤 针刺 热痛阈 睡眠时间 胃黏膜损伤指数 5-羟色胺 多巴胺 大鼠 stress induced gastric mucosal injury acupuncture heat pain threshold sleeping time gastric mucosal ulcer index 5-HT DA rats
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