大鼠力竭运动肌细胞膜Na^+-K^+-ATP酶活性与自由基代谢研究

Investigate the Active Degree of Na^+-K^+-ATPase and Free Radical Metabolism Changes of Triceps of Rats Subjected Exhaustive Exercise

目的:通过离心运动方式创建大鼠前肢一次性力竭损伤模型,观察大鼠前肢肱三头肌细胞膜Na+-K+-ATP酶活性变化,探究力竭离心运动损伤诱发的骨骼肌细胞膜结构变化,以及导致该种变化的相关机制。方法:采用大鼠下坡一次性力竭离心跑的损伤模型,研究了大鼠前肢肱三头肌骨骼肌细胞膜Na+-K+-ATP酶活性在运动后不同时间段的变化情况。结果:Na+-K+-ATP酶活性运动后即刻较安静时显著性下降,且达到峰值;即刻后开始恢复,至运动后24小时恢复明显;运动后24小时至第5天细胞膜Na+-K+-ATP酶活性值上升无显著性趋势。结论:结合同一实验体系下关于自由基代谢情况的研究,运动后即刻Na+-K+-ATP酶活性显著性降低可能与自由基损伤有关。

Objective： By using the experimental model of acute incremental exercise,To observe the active degree of Na^＋-K^＋-ATPase changes of triceps of Rats, and to discuss the effects of exhaustive exercise on cell membrane permeability and mechanism changing cell membrane permeability deeply. Methods： To create exhaustive exercise injury model by making rat ran downhill,and Na^＋-K^＋-ATP activity changes of the forelimb triceps skeletal muscle cell membrane has been studied in different time periods after exercise in rats. Results： Exhaustive exercise causes the drop of Na^＋-K^＋-ATP Enzyme markedly at a moment＇s notice after exercise, and get right at 24 hours. There was no significant trend about membrane Na^＋-K^＋-ATP value increased activity between 24 hours to 5 days after the exercise. Conclusions：Comparing studies about free radicals immediately after exercise of the same experimental system, the significantly reducing of Na^＋-K^＋-ATP activity may be damaged by free radical.