溶血磷脂酰胆碱酰基转移的调控机理研究

Regulation mechanism of acyl migration of lysophosphatidylcholine

通过对磷脂酶A1(Lecitase Ultra)酶解磷脂酰胆碱(PC)过程进行分析,证实了Sn-2-溶血磷脂酰胆碱(Sn-2-LPC)存在酰基转移现象。依据前人对单甘酯自动酰基转移机理的研究,推测了Sn-2-LPC在酸、碱条件下自动酰基转移的机理,并利用HPLC-RID确定了Sn-2-LPC可以酰基转移生成Sn-1-LPC,而Sn-1-LPC不存在酰基转移现象。同时,以硼酸为例,推测Al(OH)3、Zn(OH)2、B(OH)33种两性氢氧化物抑制Sn-2-LPC酰基转移的机理,确定了3种抑制剂的最小添加量分别为0.97、0.85、0.72 mg/m L。为合成酰基磷脂混合物,以及解释产生混合酰基磷脂的生物合成途径(溶血磷脂中间体)提供了理论基础和数据支撑。

Acyl migration phenomenon of Sn-2-lysophosphatidylcholine（Sn-2-LPC） was confirmed through hydrolysis process analysis of phosphatidylcholine by phospholipase A1（Lecitase Ultra）. Based on previous studies on the automatical acyl migration mechanism of monoglyceride,the automatical acyl migration mechanism of Sn-2-LPC in the acid and alkali conditions was speculated. Acyl migration directionalities of Sn-2-LPC and Sn-1-LPC were explored by HPLC-RID. It was found that acyl migration of Sn-2-LPC to Sn-1-LPC happened,while acyl migration of Sn-1-LPC was not present.Meanwhile,the inhibition mechanism of acyl migration of Sn-2-LPC through Al（OH）3,Zn（OH）2and B（OH）3was speculated by boric acid. The minimum dosages of Al（OH）3,Zn（OH）2and B（OH）3were determined as 0. 97,0. 85 mg / m L and 0. 72 mg / m L. A theoretical basis and data support for the synthesis of mixed acyl phospholipids and the explanation of biosynthetic pathway（lysophospholipid intermediate） of mixed acyl phospholipids were provided.