植物甾醇酯和葛根素联合使用对高脂血症小鼠降血脂作用研究

Lipid-lowering effect of combined use of phytosterol ester and puerarin in hyperlipidemia mice

研究植物甾醇酯和葛根素联合使用时对高脂血症小鼠降血脂的作用。昆明种小鼠饲喂高脂饲料,建立小鼠高脂血症模型。将建模成功的小鼠,按总胆固醇及体重分为5组,即高血脂模型组、植物甾醇酯组、葛根素组、植物甾醇酯和葛根素联合组、辛伐他丁组。对小鼠饲喂高脂饲料的同时连续灌胃5周不同剂量的植物甾醇酯、葛根素、辛伐他丁。实验结束后测定各组小鼠的体重,动脉硬化指数(AI),肝脏系数(HI),血清中的甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)。结果表明:与高血脂模型组相比,植物甾醇酯和葛根素联合组TG、TC、LDL-C、AI、HI显著降低(P<0.05),分别下降24.22%、28.01%、21.17%、67.68%、15.34%,HDL-C显著升高45.51%(P<0.05);植物甾醇酯和葛根素联合使用比单独使用时能降低高血脂小鼠TG、TC、LDL-C、AI、HI,其中部分差异显著。说明植物甾醇酯和葛根素联合使用时具有降低血脂作用,而且比两者单独使用时具有更好的治疗高脂血症作用。

The function of combined use of phytosterol ester and puerarin on lipid - lowering of hyperlipidemia mice was studied. Kunming mice were fed with high -fat diet, and hyperlipidemia mice model was established. The successful modeling mice were divided into 5 groups according to total cholesterol and weight： hyperlipidemia model group, phytosterol ester group, puerarin group, combination group of phytosterol ester and puerarin, simvastatin group. The mice were fed with high - fat diet and administrated for 5 weeks with different doses of phytosterol ester, puerarin and simvastatin. The weight, atherogenic index （ AI）, hepatic index （ HI ）, triglyceride （ TG）, total cholesterol （ TC ）, high density lipoprotein cholesterol （ HDL - C） and low density lipoprotein cholesterol （ LDL - C） of mice were detected. The results showed that compared with hyperlipidemia model group, the TG, TC, LDL - C, AI and HI of the combination group of phytosterol ester and puerarin significantly decreased （ P 〈 0.05 ） by 24. 22%, 28.01% , 21.17% ,67.68% and 15.34% respectively,while HDL - C significantly increased by 45.51% （P 〈 0.05 ）. The combined use of phytosterol ester and puerarin could reduce the TG, TC, LDL - C, AI and HIof hyperlipidemia mice than single use, and there were significant differences in some indexes. It was declared that the combined use of phytosterol ester and puerarin could efficiently reduce blood lipid and had better effect on the treatment of hy- perlipidemia than single use.