抗灰霉病解淀粉芽孢杆菌的发酵条件优化

Optimization of Fermentation Conditions of an Anti-Botrytis cinerea Bacillus amyloliquefaciens

【目的】研究灰霉病拮抗菌K1的发酵条件。【方法】以OD600值为指标采用响应面法对其发酵培养基、发酵条件进行优化。通过Plackett-Burman实验确定酵母膏、p H、接种量为其最显著的3个因素。经过BoxBehnken实验和响应面实验得到最优发酵条件。【结果】最优发酵培养基为:淀粉1%,酵母膏1.8%,硫酸钾1.5%。最优发酵条件:p H6.8,接种量为2.8%,温度25℃,转速130 r/min,装液量100 m L/250 m L。【结论】K1发酵16 h后的OD600值可达到1.490,远高于优化前0.864,明显提高了K1的生长速率。优化后发酵液抑菌直径为49mm,高于优化前的43 mm。

[Objective] Research on the fermentation conditions to K1, the antagonistic bacteria inhibiting Botrytis cinerea. [Methods] Fermentation medium and fermentation conditions were optimized in OD600 index usingresponse surface methodology. By the Plackett-Burman experiment the most significant three factors were yeast ex-tract, p H, inoculum. After Box-Behnken experiment and response surface methodology, the optimum fermentationwas determined. [Results] The optimum fermentation medium was 1% starch, 1.8% yeast extract, 1.5% K2SO4. Theoptimal fermentation conditions were as follow： p H 6.8, inoculation amount 2.8%, temperature 25 ℃, rotation speed130 r/min, liquid volume of 100 m L/250 m L. [Conclusion] The OD600 of K1 was 1.490 after 16 h fermentation. It wasmuch higher than 0.864, the OD600 before optimization. The grow rate of K1 was increased significantly. Inhibitionzone diameter was 49 mm. It was higher than 43 mm, the inhibition zone diameter before optimization.