思茅松种子园遗传多样性的RAPD分析

RAPD Analysis of Genetic Diversity of Pinus kesiya var.langbianensis from Seed Orchard

以思茅松无性系种子园收集的来自7个居群的85个思茅松用材林优良无性系为试验材料,使用2×Taq PCR Master Mix并利用Touchdown PCR构建思茅松RAPD-PCR反应体系。从120条引物中共筛选出适用于思茅松遗传多样性分析的20条引物进行PCR扩增,来自不同种源地7个居群的多态位点百分率PPB平均值59.48%;平均每个位点的有效等位基因数Ne平均值1.350 3;Nei’s基因多样指数H平均值0.206 3;Shannon’s多样性信息指数I平均值0.310 0。通过对7个居群的遗传距离进行分析,并构建UPGMA聚类树状图,从聚类结果看,7个居群可以划分为遗传差异较明显的3大类群。

As the study materials,85 Pinus kesiya var. langbianensis clones of 7 populations were collected from the clonal seed orchard. By adopting the method of 2 × Taq PCR Master Mix amplification and Touchdown PCR,RAPD reaction systems was tested. Twenty primers with high stability and polymorphism were screened out from120 RAPD primers which could be used for all samples of RAPD-PCR amplification. The results showed that,for the 7 population level,the percentage of polymorphic bands average was 59. 48 %,the number of effective alleles per band Ne average was 1. 350 3,the Nei＇s gene diversity index H average was 0. 206 3,the Shannon＇s diversity index I average was 0. 310 0. The genetic distance was analyzed and UPGMA were clustered at the 7 population,and the study results revealed these 7 population could be divided into 3 groups.