处理甜菜加工副产物微生物燃料电池阳极生物膜群落多样性研究

Diverse Microbial Communities in Microbial Fuel Cells with Sugar Beet Residue as Substrate

[目的]分析处理甜菜加工副产物微生物燃料电池(Microbial fuel cell,MFC)阳极生物膜群落多样性。[方法]以不同处理方式下的甜菜渣作为底物,以MFC为研究装置,考察其产电性能,并通过454焦磷酸测序技术分析不同处理MFC中形成阳极群落结构差异,探讨其运行的可行性、稳定性。[结果]不同甜菜渣预处理方式对MFC电能输出有影响,以碱处理和未处理的甜菜渣产生的电压高于酸处理。16S rRNA基因的焦磷酸测序结果显示,3个样品中共有14个门,其中变形菌门为优势门。由聚类分析可知,酸处理与未处理和碱处理的微生物群落构成差异明显。微生物群落中存在多种产酸菌(Paludibacter、Petrimonas以及Spirochaeta等)和产电菌(主要为Geobacter属),它们之间存在种群互养关系。产酸菌能够发酵可溶性糖或者降解纤维素产生有机酸,产电菌可以利用这些有机酸直接产电。[结论]研究证实,甜菜渣不同的处理方式导致阳极群落多样性存在差异,而阳极群落结构的不同影响微生物燃料电池电能的输出。

[ Objective ] To analyze diverse microbial communities in microbial fuel ceils with sugar beet residue as substrate. [ Method ] With sugar beet residue under different processing ways as substrate, MFC as research device, the electricity production was investigated. Using 454 pyrosequencing, differences in the community structure were analyzed, the feasibility and stability was discussed. [ Result] The research found that sugar beet residue with the different treatment as the substrates imfluence on the electricity generation of MFC. The voltage produced by MFCs with the raw and alkaline treatment was higher than the reactor by the acid treatment. We investigated the microbial community structures in anode biofihns of MFCs. By analyzing pyrosequencing sequences from the bacterial 16S rRNA gene, we demonstrated that the microbial communities were classified into 14 phyla from three anode biofilm. Hierarchical cluster analyses indicated a clear distinction among three substrates in microbial community structure. Diverse acidogenic bacteria and exoelectrogens were detected in MFCs, and there was syntrophic relatiunship among bacteria. Diverse acid-producing bacteria can ferment soluble sugar or cellulose to produce organic acids. The exoelectrogens can use them as the electron donor to directly produce the current. [ Conclusion] The results provide evidence that sugar beet residue with the different treatments can led to the diversity difference of the anode community. And the structure of the anode community can affect electrical output of MFC.