阴离子淀粉微球吸附姜黄素工艺的研究

Optimization of Adsorption of Anionic Starch Microspheres Curcumin

[目的]分析优化阴离子淀粉微球吸附姜黄素的工艺条件。[方法]以姜黄素作为载药体,研究响应面法优化阴离子淀粉微球吸附姜黄素吸附量的最佳工艺条件。通过单因素试验和Plackett-Burman试验确定了乳化剂用量、交联剂用量、三聚磷酸钠用量和浸泡时间4个主要因素对阴离子微球吸附姜黄素的影响,根据中心组合设计原理采用4因素3水平的响应面分析法,获得阴离子淀粉微球吸附姜黄素的最佳工艺条件。[结果]试验表明,阴离子淀粉微球吸附姜黄素的最佳条件为:乳化剂用量0.599 g,交联剂的用量为3.001ml,三聚磷酸钠用量为0.8 g,浸泡时间为8.5 h,姜黄素吸附量预测值为2.332 mg/g,验证值为2.367 mg/g,与预测值的相差0.035 mg/g。[结论]研究可为阴离子淀粉微球的载药性能和开发利用以及淀粉的深加工开发利用提供依据。

[Objective] To optimize technique conditions for anionic starch microsphere adopting curcumin. [Method] With curcumin as medi- cine carrier, the optimal technique conditions for nionic starch microsphere adopting cureumin were obtained by response surface method. The single factor experiments and Placket-Burman test suggested that emulsifier dosage, crosslinker dosage, values of sodium tripolyphosphate amount and soaking time were the major factors on adsorption of curcumin. Three-level response surface methodogy based on the principle of central composite design was employed to analyze the significance and interactions of the above factors by mathematical modeling. [ Result ] The results showed that the optimum adsorption of starch microspheres curcumin content were as follows ： emulsifier dosage was 0.599 g, crosslinker dosage was 3. 001 ml, values of sodium tripolyphosphate amount O. 8 g and soaking time 8.5 h. The predicted adsorption yield was 2. 332 mg/g, while the actual adsorption yield was 2.367 mg/g, with relative error of 0. 035 mg/g. [ Conclusion] The study can provide basis for development and utilization of anionic starch microsphere and deep processing of starch.