泰安市2013年手足口病病原体检测结果分析

Analysis and pathogenic diagnosis of the hand-foot-mouth diseases of specimens in Taian in 2013

目的对2013年泰安市手足口病病例进行病原体检测和分析。方法对临床诊断为手足口病病例的粪便标本505份,用实时荧光定量PCR筛选出肠道病毒通用引物阳性的标本,再分别使用Cox A 16和EV71型的特异引物进行分类检测。结果标本总肠道病毒阳性率为95.05%（408/505）,Cox A 16阳性率为31.09%（157/505）,EV71阳性率为27.92%（141/505）,其他肠道病毒阳性率为36.24%（183/505）,Cox A 16的阳性率高于EV71。发病年龄多为1-6岁,男性多于女性。结论 2013年度泰安市的手足口病疫情以EV71和Cox A 16并发为主,采用分子生物学方法检测手足口病的病原体,对手足口病的监测及防控具有重要作用。

Objective： To analyze the pathogenic diagnosis of the hand-foot-mouth diseases（ HFMD） in Taian in 2013.Methods： 505 feces specimens from clinically-diagnosed HFMD patients were collected,Pan-enterovirus was detected by fluorescent PCR,Coxsackie A16（ Cox A16） and Enterovirus 71（ EV71） were determined by real-time PCR with speciesspecial primers. Results： Positive rates of Pan-enterovirus,Cox A16 and EV71 were 95. 05%（ 408 /505）,31. 09%（ 157 /505）,and 27. 92%（ 141 /505） respectively,and other enterovirus was 36. 24%（ 183 /505）. The positive rate of Cox A16 was higher than that of EV71. High positive rate was found in 1 ~ 6 years old children and male patients. Conclusion：EV71 and Cox A16 is the dominant pathogen of the HFMD in Taian in 2013. Molecular biological methods of pathogenic diagnosis plays an important role in the surveillance and control of HFMD.