白英中甾体类总生物碱的含量测定研究

Determination of steroidal total alkaloids content in Solanum lyratum Thumb.

目的:建立白英中甾体类总生物碱含量的测定方法。方法:采用醇提-酸醇水解法提取白英中的总生物碱,分离得到甾体类总生物碱,用紫外-可见分光光度法,以澳洲茄胺为对照,溴甲酚绿为显色剂,在412 nm处测定吸光度值,计算白英中甾体总生物碱的含量。结果:澳洲茄胺在1.6μg/m L^9.6μg/m L范围内与吸光度值呈良好的线性关系(r=0.999 5),平均回收率为88.70%~102.2%(n=9),RSD为3.09%~4.96%,不同来源的白英药材中甾体总生物碱的含量有一定的差异。结论:该方法准确、简单、灵敏,适合测定白英药材中甾体类总生物碱的含量,并且可为白英的质量评价提供理论参考。

Objective：To establish the assay method of steroidal total alkaloids content in Solanum lyratum Thumb..Methods：After extracting total alkaloids by the method of ethanol extracting-hydrolyzing with acid and ethanol,steroidal total alkaloids were isolated. Then its absorbance at the point of 412 nm was observed by the ultraviolet-visible spectrophotometry and its content was calculated. Results：The solasodine curve was linear over the range of 1.6 μg/m L -9.6μg/m L with the correlation coefficient of 0.999 5. The average recovery rate were 88.70%-102.2%（RSD=3.09%-4.96%,n=9）.The contents of steroidal alkaloids in Solanum lyratum Thumb. which was from different samples had statistically difference.Conclusion：The method is accurate,simple,sensitive and reproducible,especially for the detection of steroidal alkaloids content in Solanum lyratum Thumb.. It can provide a theoretical reference for the quality evaluation of Solanum lyratum Thumb..