柴胡疏肝散对衣霉素诱导内质网应激模型细胞活性的影响

Observation of the Effect of Chaihushugansan on the Cell Activities of Endoplasmic Reticulum Stress Cells Induced by Tunicamycin

目的:探讨柴胡疏肝散含药血清对衣霉素诱导的内质网应激(ERS)细胞模型NG108-15细胞活性的影响。方法:采用衣霉素(TM)孵育NG108-15细胞,诱导ERS细胞模型,并将诱导后的细胞分成五组,模型组、安理申西药组、柴胡疏肝散高、中、低剂量组,分别采用正常空白血清、安理申含药血清、高、中、低剂量柴胡疏肝散含药血清进行干预,同时设置正常空白血清孵育的未经TM诱导的正常组。观察各组细胞的活性。结果:柴胡疏肝散高、中、低剂量组,细胞的活性明显高于模型组细胞的活性(P<0.05);西药安理申组和柴胡疏肝散高剂量组细胞的活性均明显高于其他各组(P<0.05),且两者之间无明显差异(P>0.05)。结论:柴胡疏肝散含药血清对正常NG108-15神经细胞没有明显毒副作用,但其可明显提高衣霉素诱导的内质网应激NG108-15细胞的生存率,其机制可能与柴胡疏肝散缓解衣霉素诱导的细胞内质网应激有关。

Objective To observe the effect of Chaihushugansan（CHSGS） on cell activity of endoplasmic reticulum stress NG108-15 cells induced by tunicamycin（TM）. Methods The ERS cell model was induced by adding TM into NG108-15 cells to incubate them together, and the incubated cells were divided into five groups including ERS model, aricept group, CHSGS groups （classified by high, middle and low concentration）, and treated with normal rabbit serum, aricept rabbit serum, CHSGS with serum intervention （classified by high, middle and low concentration） respectively. And the cell activity in the classified groups was observed and discussed. Results The cell activity for CHSGS groups showed significantly better results than the model group （P 〈 0.05）; the cell activity for aricept group and high concentration CHSGS group showed better results than other groups （P 〈 0.05）, and no obvious difference was found between the two groups （P 〉 0.05）. Conclusions The results show that an ERS model can be formed by TM induced NG108-15 neural cells, CHSGS can be used to improve the cells＇ activity and increase the cells＇ survival rate without any side effects in the whole process. The mechanism for this phenomenon may be related to the CHSGS for releasing the endoplasmic reticulum stress.