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Haplotypic Variation of Chrysoperla externa (Hagen) (Neuroptera: Chrysopidae) in Different Geographic Locations

Haplotypic Variation of Chrysoperla externa (Hagen) (Neuroptera: Chrysopidae) in Different Geographic Locations
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摘要 Chrysoperla externa is found in environment, their populations may experience several Brazilian agroecosystems and feeds on many agricultural pests. In each different environmental pressures that can favor the emergence of genetic mutations and interfere in insect's ecological plasticity. Such genetic phenomena can be observed through the use of molecular markers, in particular mitochondrial genes, which are easy to handle and extract. The aim of this work was to study haplotype diversity of Chrysoperla externa in the cities of Jaboticabal (SP), Brotas (SP), Rifaina (SP), Sao Carlos (SP), Sao Jose dos Campos (SP) and Sao Sebastiao do Paraiso (MG), and evaluate the degree of genetic differentiation between these populations. Two mitochondrial genes were sequenced to compare these populations. It was found that there were eight haplotypes, six mutations for cytochrome c oxidase subunit 1 (COl) gene and a greater haplotype diversity was found in Brotas and Sao Sebastiao do Paraiso. There were four mutations and six haplotypes for 16S gene and the highest haplotype diversity was found in the city of San Sebastiao do Paraiso. No pattern of haplotype distribution for the populations analyzed was found. The genetic distance between populations of C. externa was not significant, showing that they are genetically compatible.
出处 《Journal of Agricultural Science and Technology(B)》 2014年第10期804-810,共7页 农业科学与技术(B)
关键词 BIOGEOGRAPHY mitochondrial DNA molecular ecology Chrysoperla externa. 单倍型 外耳炎 地理位置 草蛉 脉翅目 细胞色素C氧化酶 线粒体基因 基因突变
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