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On-column Refolding of Diphtheria Toxin Variant CRM197 by Different Metal-Chelating Affinity Chromatography Matrices

On-column Refolding of Diphtheria Toxin Variant CRM197 by Different Metal-Chelating Affinity Chromatography Matrices
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出处 《Journal of Chemistry and Chemical Engineering》 2014年第12期1135-1141,共7页 化学与化工(英文版)
关键词 金属螯合亲和层析 白喉毒素 矩阵 复性 变异 交叉反应 大肠杆菌 CRM Refolding, chromatographic matrix, cross-reacting material 197, metal-chelating affinity chromatography
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  • 1Giannini, G.; Rappuoli, R.; Ratti, G. The Amino-Acid Sequence of Two Non-toxic Mutants of Diphtheria Toxin: CRM45 and CRM 197. Nucleic Acids Res. 1984, 12, 4063-4069.
  • 2Uchida, T.; Pappenheimer, A. M. Jr; Greany, R. Diphtheria Toxin and Related Proteins. I. Isolation and Properties of Mutant Proteins Serologically Related to Diphtheria Toxin. 1. Bioi. Chem. 1973,248,3838-3844.
  • 3Shinefield, H. R. Overview of the Development and Current Use of CRM 197 Conjugate Vaccines for Pediatric Use. Vaccine 2010, 17, 4335-4339.
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  • 7Rappuoli, R. Isolation and Characterization of Corynebacterium diphtheriae Nontandem Double Lysogens Hyperproducing CRMI97. Appl. Environ. Microb. 1983, 46, 560-564.
  • 8Bishai, W. R.; Miyanohara, A.; Murphy, J. R. Cloning and Expression in Escherichia coli of Three Fragments of Diphtheria Toxin Truncated within Fragment B. J Bacteriol. 1987,169,1554-1563.
  • 9Spilsberg, B.; Sandvig, K.; Wiilchli, S. Reconstitution of Active Diphtheria Toxin Based on a Hexahistidine Tagged Version of the B-Fragment Produced to High Yields in Bacteria. Toxicon 2005, 46, 900-906.
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