慢性扁桃体炎组织活检T淋巴细胞亚群研究

The research change of T cell subset in chronic tonsillitis

目的探寻反映慢性扁桃体炎患者的客观免疫指标。方法91例住院慢性扁桃体炎患者和18例正常健康志愿者，术前均取样双侧扁桃体组织；进行免疫荧光分析。用SPSS13．0进行统计，计数资料用Х^2检验，计量资料用T检验。结果与健康对照组比较，滤泡边缘及冠区的CD4＋T细胞与CD8^＋T细胞比值，健康对照组为3：1，慢性炎性扁桃体组为1：1。结论CD4＋T细胞与CD8＋T细胞比率的变化提示局部细胞免疫起了一定作用。

Objective we attempted to determine a potential relationship between the surgical indication and laboratory tests regard to chronic tonsillitis. Methods A total of 91 patients with chronic tonsillitis and 18 healthy volunteers were studied from the Clifford Hospital of Guangzhou TCM University. We had collected specimens from bilateral tonsils before operation and then assessed through a series of biopsy, electron microscopy, immunofluorescence, immunohistochemistry and so on. The data were analysed by using the SPSS 13. 0 statistical software package for Windows. Results Contrast to healthy volunteers, bacterial culture of tonsil surface was not significant difference（P〉0.05）, while germiculture of tissue biopsy was significant difference（P〈0.05）. Under electron microscopy,obviously abnormal keratosis of crypt epithelial cells and bacteria could be seen in the chronic tonsillitis group. On transmission electron microscopy, neutrophile granulocyte and non neutrophile granulocyte could be find between the crypt epithelial cells, even coccie bacteria could be encuntered in the crypt epithelial cells. The ratio of CD4＋ T cells to CD8＋ T cells is 3：1 in the control group and 1：1 in the chronic tonsillitis group, analyzing data from specimens of follicle and coranal. Comparing to control group, there were different degrees of keratosis in the chronic tonsillitis group. Conclusion Germiculture of tonsilar biopsy was a feasihle method to investigate the pathogeny of chronic tonsillitis; electron microscopy eorroboratesd the results of germiculture of biopsy, finding obviously abnormal keratosis of crypt epithelial cells and pathogenic bacteria; the ratio of CD4＋ T cells to CD8＋ T cells demonstrated cellular immunity playing an important role in developement of chronic tonsillitis; research of keratin authenticatesd different degree of keratosis of chronic tonsillitis.