不同菌群对黄姜废渣厌氧消化产沼气的性质研究

Research of Characters on Biogas Production of the Yam Residue Anaerobic Digestion in Different Bacteria

该研究以探索不同菌群对黄姜废渣厌氧消化产沼气的性质为目的,分别以酵母废水活性污泥和实验室驯化的秸秆分解产甲烷菌群为产沼气菌源进行发酵,通过监测厌氧发酵过程中的甲烷生产效率、纤维素酶活性等指标,评价黄姜废渣厌氧发酵产甲烷的能力,同时考察纤维素分解菌群WDC2的加入对黄姜废渣产沼气的影响。该研究对有效处理黄姜皂素生产废渣、促进区域环境安全具有重要意义。结果表明:黄姜废渣在产甲烷菌群的作用下均能生成大量沼气,最高日产气量为可达到2 701 m L/d,最大产气效率为855 m L/g。酵母废水活性污泥产气效率明显优于秸秆分解产甲烷菌群。加入WDC2菌群能显著提高厌氧消化的前期纤维素酶活力,最高酶活分别为达到1.22 U/m L和9.42 U/m L,但WDC2的加入并没有对发酵体系的产甲烷效率产生明显的促进作用。

This study explored the characteristics of bacteria producing biogas by anaerobic digestion in yam waste. Bacteria source, respectively from activated sludge of yeast waste water and straw decomposition methanogenic bacteria by laboratory acclimating, were fermentated continuously for 58 days to study the biogas production. While, the influence of adding cellulose-decomposing WDC2 bacteria were investigated. The indicators of methane production efficiency and cellulase activity were monitored to evaluate the ability of anaerobic fermentation producing methane in sapogenin waste. The results showed that methanogenic bacteria could produce large amounts of methane in turmeric waste and the highest daily gas production was up to 2 701 mL, the maximum gas production efficiency was 855 mL/g. Meanwhile, the gas production efficiency of bacteria from yeast wastewater sludge was much better than straw decomposition methanogenic bacteria. Adding WDC2 bacteria could significantly improve the cellulase activity of anaerobic digestion process early. The cellulase activity and xylanase activity were the highest 1.22 U/mL and 9.42 U/mL. But adding WDC2 bacteria had no effect on the efficiency of the production of methane fermentation system.