藏族药翼首草不同药用部位UFLC指纹图谱比较

UFLC Fingerprints of Different Medicinal Parts of Pterocephali Herba

目的:建立藏族药翼首草不同药用部位的指纹图谱,并比较翼首草地上、地下部位指纹图谱的差异。方法:采用Agilent Proshell 120 SB-C18色谱柱(4.6 mm×100 mm,2.7μm),以乙腈-0.2%磷酸水溶液为流动相,梯度洗脱,流速1.0 m L·min-1,检测波长238 nm,柱温30℃。结果:建立了17个批次翼首草地上、地下部位的指纹图谱,地上部分共提取了15个色谱峰,其中6,7号峰为地下部分缺失峰,并结合对照品对其中的5个色谱峰进行了指认,分别为绿原酸、马钱苷、獐牙菜苷、吴茱萸苷、大花双参苷A。相似度结果表明,地下部分相似度较好,均>0.9;地上部分第2,3,7,11,16,17批次相似度<0.9,其他批次均>0.9。结论:该方法稳定、可靠、重复性好,可用来控制翼首草的质量。

Objective： To establish an UFLC-PDA method for the fingerprint of Pterocephali Herba, and compare the fingerprints of different medicinal parts. Method： The separation was performed on an Agilent Proshell 120 SB-C18 （4.6 mm×100 mm, 2.7 μm） using gradient elution with water-formic acid （ 100： 0.2） and acetonitrile, at a flow rate of 1.0 mL ·min^-1. The column temperature was kept at 30 ℃ , and detection wavelength was set at 238 nm. Result： The UFLC-PDA fingerprints of 17 batches were set up. The results showed that 15 chromatographic peaks were extracted as the common peaks of fingerprint of aboveground part, while in underground part peak 6 and peak 7 were nonexistent. 5 peaks were identified as chlorogenic acid, loganin, sweroside, evodia rutaeearpa glycosides and triplostoside A. The similarity degrees were above 0.9 excepted 6 batches of aboveground part. Conclusion： The method has good presicion, stability and repeatability that it could provide basis for quality control and evaluation of Pteroeephali Herba