HPLC同时测定补阳还五汤提取液中4种成分的含量

Simultaneous Determination of Four Components in Buyang Huanwu Tang Extract by HPLC

目的：建立HPLC同时测定补阳还五汤提取液中苦杏仁苷、芍药苷、毛蕊异黄酮葡萄糖苷、阿魏酸含量的方法。方法：采用反相高效液相法,依利特Hyperil ODS2色谱柱,流动相乙腈-0.1%磷酸水梯度洗脱,流速1.0 m L·min^-1,检测波长分别为210（苦杏仁苷）,230（芍药苷）,250（毛蕊异黄酮葡萄糖苷）,315 nm（阿魏酸）。结果：苦杏仁苷、芍药苷、毛蕊异黄酮葡萄糖苷、阿魏酸线性范围分别为1.080~10.80,1.735~17.35,0.242~2.42,0.106~1.06μg,相关系数分别为0.999 8,0.999 8,0.999 5,0.999 6;平均加样回收率分别为98.39%,98.89%,97.25%,95.39%,RSD分别为1.9%,2.0%,3.3%,2.6%。结论：该建立的高效液相色谱法方便、简便、准确,适用于同时对补阳还五汤提取液中苦杏仁苷、芍药苷、毛蕊异黄酮葡萄糖苷及阿魏酸的含量测定。

Objective： An HPLC method for simultaneous determination of four components in Buyang Huanwu Tang extract was established. Method： The Buyang Huanwu Tang extract was separated on an Hyperil ODS2 column by gradient elution using acetonitrile （A） -0. 1% phosphate （B） as the mobile phase. The flow rate was 1.0 mL ·min^-1 The detection wavelengths were 210 （amygdalin）, 230 （paeoniflorin）, 250 （campanulin） , 315 nm （ferulic acid）. Result： The linear ranges of amygdalin, paeoniflorin, campanulin and ferulic acid were 1. 080-10.80 （r =0. 999 8） , 1. 735-17.35 （r =0. 999 8） , 0. 242-2.42 （r =0. 999 5） , 0. 106- 1.06 （ r = 0. 999 6） μg, respectively; the average recovery was 98.39% （ RSD 1.9% ）, 98.89% （ RSD 2.0% ） , 97.25% （RSD 3.3% ） , 95.39% （RSD 2.6% ）. Conclusion： The method is rapid and accurate for simultaneous determination of amygdalin, paeoniflorin, campanulin and ferulic acid in Buyang Huanwu Tang extract.