不同配伍配比对麻黄-白术药对有效成分含量的影响

Changes of Chemical Constituents in Water Extracts of Ephedrae Herba with Atractylodis Macrocephalae Rhizoma in Different Compatibility Ratio

目的:研究麻黄-白术药对不同配伍、配比下水煎液中有效成分含量的变化,探求麻黄-白术药对的化学变化规律。方法:采用GC-MS考察麻黄水煎液及麻黄-白术药对不同配比共煎液中麻黄类生物碱(麻黄碱、伪麻黄碱、去甲基麻黄碱、去甲基伪麻黄碱和甲基麻黄碱)含量的变化情况;采用HPLC考察白术水煎液及麻黄-白术药对不同配比共煎液中白术内酯Ⅰ,Ⅱ,Ⅲ的含量变化情况。结果:与麻黄单煎液相比,麻黄-白术(3∶2,3∶4,3∶8)3种比例配伍后,随着白术的配伍比例增大,水煎液中5种麻黄类生物碱含量均有所下降(P<0.05)。与白术单煎液相比,麻黄-白术配伍后,麻黄-白术(3∶2,3∶4,3∶8)3个配比组水煎液中白术内I,Ⅱ,Ⅲ的含量均显著降低(P<0.01)。结论:麻黄与白术配伍后,对彼此有效成分的溶出有一定的抑制作用。

Objective： To study the changes of chemical constituentsin water extracts of couplet medicines Ephedrae Herba and Atractylodis Macrocephalae Rhizomain different compatibility ratio, and to search the compatibility rule of this couplet medicines. Method： The content of five alkaloids, norpseudoe phedrine, norephedrine, ephedrine, pseudoephedrineand methyle phedrine were determined by GC-MS. The content of atractylenolide Ⅰ , atractylenolide Ⅱ, and atractylenolide Ⅲ were determined by HPLC. Result： The three compatibility ratios of Ephedrae Herba and Atractylodis Macroce phalae Rhizoma were 3：2, 3：4, and 3：8. Compared with decoction of Ephedrae Herba, the content of five alkaloids were decreased （P 〈 0.05） along with the increasein the proportion of Atractylodis Macrocephalae Rhizoma. And the content of atractylenolide Ⅰ , atractylenolide Ⅱ, atractylenolide Ⅲ were also decreased （P 〈 0.01 ）. Conclusion： Combined Ephedra Herba with Atractylodis Macrocephalae Rhizoma, the dissolution of compounds in water extracts was inhibited with each other.