X染色体连锁凋亡抑制基因小分子抑制剂Embelin对宫颈癌细胞株HeLa细胞凋亡的影响及分子机制

Effect of Embelin on apoptosis of He La cells and the molecular mechanism

目的:研究X染色体连锁凋亡抑制基因(XIAP)小分子抑制剂Embelin在体外对宫颈癌细胞株He La生长的影响,并探讨其作用机制。方法:应用CCK-8检测不同浓度Embelin对宫颈癌细胞株He La增殖的影响;利用流式细胞仪检测Embelin对He La细胞凋亡的影响;Western blot方法检测Embelin作用后细胞XIAP、Caspase-3、Caspase-9表达的变化。结果:Embelin对宫颈癌细胞株He La增殖具有显著抑制作用,差异有统计学意义(P<0.05);经不同浓度Embelin处理后,He La凋亡率明显增高,与对照组比较差异有统计学意义(P<0.01);经Embelin作用24 h后,He La细胞Caspase-9,Caspase-3,PARP被激活,表达增加。结论:Embelin在体外可显著抑制He La细胞增殖,诱导细胞凋亡,其机制可能是通过拮抗XIAP的作用,激活Caspase依赖性的细胞凋亡途径。

Objective： To study the effect of X-linked inhibitor of apoptosis （XIAP） Embelin on growth of cervical cancer HeLa cells in vitro, explore the mechanism. Methods： CCK-8 was used to detect the effects of different concentrations of Embelin on proliferation of cervical cancer HeLa cells; flow cytometry was used to detect the effect of Embelin on apoptosis of cervical cancer HeLa cells ; Western blot was used to detect the changes of XIAP, Caspase-3, and Caspase-9 expressions in cervical cancer HeLa cells after treated with Embelin. Results： Embelin significantly inhibited the proliferation of cervical cancer HeLa cells （ P〈0.05 ） . The apoptotic rate of cervical cancer HeLa cells significantly increased after treated with different concentrations of Embelin, compared with control group, there was statistically significant difference （P〈0.01 ） . Treating cervical cancer HeLa cells with Embelin after 24 hours, Caspase-9, Caspase-3, and PARP were activated, and their expressions increased. Conclusion： Embelin can significantly inhibit proliferation and induce apoptosis of cervical cancer HeLa cells in vitro, which may take effect by antagonizing XIAP and activating Caspase-dependent apoptotic pathway.