纤维素酶联合超声波提取沙溪蜜柚果皮中的果胶

Combined Extraction of the Pectin from Shaxi Honey Pomelo Peel by Ultrasonic-assisted Cellulase

采用纤维素酶联合超声波提取了沙溪蜜柚果皮中的果胶.研究了料液比、酶用量、酶解时间、酶解温度、提取pH、超声波功率、超声波提取时间、超声波提取温度对果胶产率的影响.以正交优化试验确定了果胶的最佳提取工艺条件:酶用量为15U/ml、料液比为1∶35、超声波时间为70min、超声波功率为320 W.在最佳工艺条件下果胶的产率为20.2%,产品半乳糖醛酸含量为83.8%,酯化度为61.6%,含水量为6.2%,水不溶灰分为2.44%,酸不溶灰分为0.29%.

The pectin was extracted from the Shaxi honey pomelo peel by ultrasonic-assisted cellulase.The influence of the solid-liquid ratio,enzyme dosage,enzymolysis time,enzymolysis temperature,extracting pH value,ultrasonic power,ultrasonic extracting time,extracting temperature,on the yield of the pectin,were investigated.The optimal extracting technological conditions were determined by orthogonal experiments,which were listed as followed：the enzyme dosage of 15U/ml,solid-liquid of 1∶35,ultrasonic time of 70 min,and the ultrasonic power of 320 W.Under the optimal conditions,the yield of pectin was 20.2%;the galacturonic acid content of the product was 83.8%;the esterification degree was 61.6%;the moisture content was 6.2%;the content of the water-insoluble ash was 2.44%;and the content of acid-insoluble ash was 0.29%.