甜菜属厚皮菜SWTY-1细胞质VNTRs多态性分析

Analysis for variable number of tandem repeats in cytoplasm genome of Beta vulgaris cicla SWTY-1

利用数目可变串联重复序列(Variable Number of Tandem Repeats,VNTRs)微卫星标记方法,对重庆厚皮菜甜菜材料SWTY-1群体中100个单株的细胞质线粒体DNA片段中TR2位点VNTRs片段多态性进行分析。结果显示97个单株线粒体TR2位点微卫星串联重复序列均为3拷贝,与普通糖甜菜一致;3个单株线粒体TR2位点微卫星串联重复序列为6拷贝,发现甜菜属厚皮菜细胞质TR2位点VNTRs存在多态性,在该群体中发现了不同于甜菜栽培种新的细胞质单株。对该群体材料100个单株的抽薹及结籽进行观测,结果显示微卫星串联重复序列为6拷贝的变异植株中2个单株花期未抽苔开花,1株抽苔晚未形成正常种子;细胞质TR2位点VNTRs片段拷贝数为3的植株中2个单株未能正常抽薹,其他植株均正常抽薹结籽。

Molecular identification for cytoplasm polymorphism of Beta vulgaris cicla L materials SWTY-1 was conducted in this paper. Polymorphisms in mt DNA TR2 locus of Beta vulgaris cicla SWTY-1 were analyzed using variable number of tandem repeats（VNTRs） molecular markers method. The result showed that the numbers of tandem repeats in mt DNA TR2 locus were three-copy array of tandem repeats in 97 single plants among the 100 plants of Swiss chard,which was consistent with sugar beet. Six-copy VNTRs in mt DNA TR2 locus were found in 3single plants among the population. Two plants could not bolt among the three plants whose VNTRs copy numbers were six. One plant bolting late could not form normal seeds among the three plants whose VNTRs copy number were six. Only two plant among the 97 plants with three-copy in TR2 locus failed to bolt normally and others bolt normally and form seeds.