问号钩体56601株外分泌蛋白功能研究

Function analysis of L. interrogans str. 56601 extracellular proteins

目的探讨体内外差异表达的问号钩体56601株外分泌脂蛋白(LA_3469、LA_2413、LA_2823和LB_194)的致病功能。方法利用体外重组表达技术,在体外表达、纯化四个外分泌脂蛋白。采用ELISA的方法,检测钩体外分泌脂蛋白与不同细胞外基质的黏附功能。结果 LA_3469与LA_2413能够与细胞外基质Laminin、Collagen I以及Collagen IV发生黏附作用,LA_3469还能够与Fibronectin发生黏附作用。结论结合前期的研究,提示问号钩体外分泌脂蛋白LA_3469可能是钩体致病过程中的一个重要的致病因子。

Objective To explore the virulence function of extraeellular lipoproteins （including LA 3469, LA_2413, LA_2823 and LB_194） from the in vivo and in vitro differentially expressed L. interrogans str.56601. Methods We constructed reeombinants in vitro for expression and purification of the four extraeellular lipoproteins. In addition, we evaluated the adhesion ability of different extracellular lipoproteins and ECMs by ELISA. Results Both LA_3469 and LA_2413 eanadhesion to Laminin, Collagen I and Collagen IV. Furthermore, LA_3469 alsoean bind to Fibronectin. Conclusion Combined the previous studies, we considered that the extraeellular lipoprotein of L.interrogans, LA 3469, could be an important virulence factor during the leptospiral process.