IL-28B基因多态性与丙型肝炎自然转归的相关性研究

Correlation of IL-28B gene polymorphism and natural outcome of hepatitis C

目的:探讨IL-28B单核苷酸多态性与丙型肝炎病毒(HCV)感染自然转归的相关性。方法:采用Sanger基因测序法检测广州地区无偿献血人群中266名HCV感染者(107名自发清除者和159名慢性感染者)和97名健康献血者IL-28B rs12979860位点基因型和等位基因,并统计分析其在HCV感染者与健康献血者,HCV慢性感染者与健康献血者,HCV自发清除者与慢性感染者中的分布差异。结果:159名HCV慢性感染者,107名自发清除HCV者和97名健康献血者多表现为CC基因型,分别占83.6%、95.3%和86.6%,CT基因型分别占16.4%、4.7%和13.4%,未发现TT基因型。HCV感染者和健康献血者,HCV慢性感染者和健康献血者CC/CT基因型分布差异均无统计学意义(χ2=0.204,P=0.652;χ2=0.406,P=0.524),但在HCV慢性感染者和自发清除HCV者中CC/CT基因型分布差异有统计学意义(χ2=8.474,P=0.004),自发清除组中CC基因型比率高于慢性感染组,自发清除组C等位基因的频率高于慢性感染组(χ2=7.949,P=0.005)。结论:在广州献血人群中,IL-28B rs12979860基因多态性与HCV的易感性不相关,但与HCV感染的自发清除相关,表现为C等位基因有利于丙型肝炎病毒(HCV)的自发清除。

Objective：To explore the association between interleukin（IL）-28 B single nucleotide polymorphisms and natural outcome of hepatitis C virus. Methods： The IL-28 B rs12979860 locus was genotyped in 266 HCV infected volunteer blood donors（107spontaneous cleared and 159 chronic infection） and 97 healthy controls using Sanger sequencing assay. The difference in rs12979860 genotypes and allele frequencies between the six groups（107 spontaneous cleared and 159 chronic infection,266 HCV infection and 97 healthy controls,159 chronic infection and 97 healthy controls） were analyzed by statistics. Results： 159 HCV chronic infection,107 spontaneous cleared and 97 healthy controls,were shown more CC genotype,accounting for 83. 6%,95. 3% and 86. 6%,respectively,while the CT genotype accounted for 16. 4%,4. 7% and 13. 4% respectively. No TT genotype was found. The CC / CT genotype was not significant difference between HCV infection and healthy controls,chronic infection and healthy controls（ χ2= 0. 204,P = 0. 652;χ2=0. 406,P = 0. 524）,but between chronic infections and spontaneous clearance had statistically significant（ χ2= 8. 474,P = 0. 004）,the frequence of C allele in spontaneous cleared was higher than HCV chronic infection（ χ2= 7. 949,P = 0. 005）. Conclusion： The gene polymorphism of IL-28 B rs12979860 is not related to HCV susceptibility,but there are differences in chronic infection and spontaneous cleared,showing the C allelic in favor of HCV spontaneous cleaed.