卵巢癌患者外周血Th17/Treg细胞的检测及其临床意义

Expression and clinical significance of Th17 and Treg cells in peripheral blood of patients with ovarian cancer

目的:检测卵巢癌患者外周血中辅助性T细胞17(T help cell 17,Th17)/调节性T细胞(regulatory T cell,Treg)的水平并探讨其临床意义。方法:选取55例卵巢癌和60例健康对照者为研究对象,采用细胞内染色流式细胞术(Flow cytometry,FCM)、实时定量PCR(real time PCR)、酶联免疫吸附法(Enzyme linked immunosorbent assay,ELISA)法检测外周血Th17/Treg细胞百分率、核转录因子retinoid-related orphan receptor gamma-t(RORγt)/foxhead winged-helix box protein 3(Foxp3)mRNA的表达以及血浆中白细胞介素17(Interleukin-17,IL-17)/转化生长因子-β1(Transforming growth factor-β1,TGF-β1)的水平。结果:卵巢癌患者外周血中CD4+IL-17+/CD4+T细胞百分率、RORγt mRNA及IL-17水平与正常对照组相比明显升高,差异有统计学意义(P<0.05)。与正常对照组相比,卵巢癌患者外周血中CD4+Foxp3+Treg/CD4+T细胞百分率、Foxp3 mRNA及TGF-β1水平明显升高,差异有统计学意义(P<0.05)。结论:卵巢癌患者外周血中Th17/Treg细胞失衡,Th17/Treg细胞可能参与卵巢癌的发病过程。

Objective：To detect the levels of T help cell 17（Th17） /regulatory T cell（Treg） in the peripheral blood of patients with ovarian cancer and explore their clinical significances. Methods： 55 patients with ovarian cancer and 60 normal controls were enrolled. The percentage of Th17 / Treg cells were detected by flow cytometry（ FCM）. The expression of retinoid-related orphan receptor gamma-t（ RORγt） / foxhead winged-helix box protein 3（ Foxp3） mRNA was measured by real time PCR and the plasma levels of IL-17 / TGF-β1 were detected by enzyme linked immunosorbent assay（ ELISA）. Results： The percentage of CD4＋IL-17＋/ CD4＋T cells,the mRNA expression of RORγt and the plasma levels of IL-17 increased significantly in patients with ovarian cancer than in normal controls（ P〈0. 05）. Compared with normal controls,the percentage of CD4＋Foxp3＋Treg / CD4＋T cells,the mRNA expression of Foxp3 and the plasma levels of TGF-β1 increased significantly in patients with ovarian cancer（ P〈0. 05）. Conclusion： The imbalance of Th17 / Treg cells may exist in the peripheral blood of patients with ovarian cancer. Th17 / Treg cells may participate the pathology of ovarian cancer.