不同培养料对真姬菇胞外酶活性的影响

Effect of Different Cultivation Substrates on Extracellular Enzyme Activities of Hypsizygus marmoreus

以真姬菇(Hypsizygus marmoreus)为试材,以五节芒(Miscanthus floridulus)和芒萁(Dicranopteris dichotoma)2种菌草、木屑和棉籽壳为主要原料栽培真姬菇,研究真姬菇菌丝胞外纤维素酶、半纤维素酶、β?葡萄糖苷酶、淀粉酶、漆酶和锰过氧化物酶在其生长发育阶段的活性变化。结果表明:无论在何种培养料中,真姬菇菌丝分泌的木质素酶类(漆酶、锰过氧化物酶)活性最大值都在菌丝后熟阶段,而非木质素酶活性最大值则都在菌丝生殖阶段;不同培养料中真姬菇菌丝分泌的同种胞外酶,其活性在各生长发育阶段不同。在变化趋势上,木质素酶类的活性趋势差异在菌丝体后熟阶段,非木质素酶类基本相似。由此说明真姬菇胞外酶活性与生长发育阶段密切相关,并且呈现阶段性变化;真姬菇胞外木质素酶类的活性及变化趋势,与其菌丝生长阶段和培养料的组成有关,非木质素酶类活性的变化趋势由真姬菇生长阶段决定,培养料的不同仅对其活性大小有影响。

Taking Hypsizygus mannoreus as materials, the extracellular enzyme activities of carboxmethycellulase hemicellulase, β-glucosidase, amylase,laccase and manganese peroxidase at different growth stages of Hypsizygus rnarmoreus were studied, with two kinds of JUNCAO formula （Miscanthus floridulus, Dicranopteris peclata ）, and two conventional formula （sawdust and cotton seed hull）. The results showed that the activity of H. marmoreus mycelium secretion of lignin enzymes （laccase, Mn-peroxidase） reached the maximum at the ripening stage of mycelium in each culture material, and the activity of non-ligninolytic enzyme reached the maximum at the mycelial reproductive stage. The activity of the same extracellular enzymes, secreted by H. marmoreus at different culture medium, were disparate in different growth period, the changing trends of lignin enzymes activity were imparity, the trend of Non-lignin enzyme activity were essentially same. The results indicated that the change of the activity of H. marmoreus mycelium secreted enzyme was closely related to growth period and periodical. The activities and changing tendency of lignin enzymes were related to the mycelial growth stage of H. marmoreus and the components of formula,meanwhile,the changing tendency of non-lignin enzymes activities of H. marmoreus were determined by the mycelium growth stage of H. marmoreus and could be affected by different formula.