1株链霉菌产ε-聚赖氨酸的结构及分子量分析

Analysis of chemical structure and mass weight of a product produced by a suspected ε-poly-l-lysine- producing Streptomyces strain

目的：研究确定1株疑似产ε-聚赖氨酸链霉菌所合成产物的化学结构和分子量。方法：采用D152树脂离子交换法分离纯化ε-聚赖氨酸;薄层色谱分析化学组成;紫外可见扫描光谱分析有机官能团;长程异核位移相关谱分析赖氨酸分子间的键连接方式;Tricine-SDS-PAGE电泳分析ε-聚赖氨酸分子量。结果：薄层色谱显示赖氨酸为纯化产物的惟一化学组成;纯化产物200nm处有最大吸收峰,260～280nm蛋白特征吸收处无吸收;长程异核位移相关谱显示纯化产物为ε-聚赖氨酸;所分离ε-聚赖氨酸分子量约为3300u左右。结论：菌株GIM8发酵产物为ε-聚赖氨酸;薄层色谱、紫外可见扫描光谱以及长程异核位移相关谱三者结合适用于ε-聚赖氨酸的化学组成和结构分析。

Objective： The aim of this study was to determinate chemical structure and mass weight of a product suspected as ε- poly- l- lysine from a Stretomyces strain.Methods： The chemical composition of the product was determined by thin layer chromatography（ TLC）; the UV- VIS wavelength scanning was used to analyze organic groups; the chemical bond among l- lysine residues was analyzed by heteronuclear multiple- bond correlation spectrum（ HMBC）.Tricine- SDS- PAGE was employed for the analysis of molecular weight.Results： The TLC profile indicated that the product consisted of only lysine; the maximum absorbance was observed at 200 nm,and almost no absorbance at 260～ 280 nm that characteristics of a protein was detected. The HMBC spectrum confirmed that the product from the strain was ε- poly- l- lysine.The ε- poly- l- lysine had an average mass weight of around 3300 u.Conclusion： The product produced by strain GIM8 was ε- poly- l- lysine,and the molecular weight was around3300 u.The combination of the TLC,UV- VIS wavelength scanning,and HMBC can be a suitable approach for the determination of the chemical structure of ε- poly- l- lysine.