不同产地绒柄牛肝菌紫外指纹图谱鉴别分析

Ultraviolet spectrum identification of Boletus tomentipes from different regions

应用紫外光谱技术建立了云南9个不同地区绒柄牛肝菌的紫外指纹图谱,采用欧氏距离和主成分分析法对230～450nm的紫外光谱数据进行分析。结果显示该方法的精密度、重现性及10h内稳定性的RSD分别在0～2.87%、0.03%～0.63%、0.04%～1.73%之间;不同产地样品间的欧氏距离值在0.26～6.52之间,样品间的欧氏距离明显;主成分分析的前三个主成分累积贡献率达到86.848%,能够表达样品主要信息,前两个主成分的二维投影图能够较好地区分不同产地绒柄牛肝菌样品。紫外光谱结合欧氏距离、主成分分析法能够快速鉴别不同产地绒柄牛肝菌。

The ultraviolet fingerprints of Boletus tomentipes from nine different areas in Yunnan were established.UV spectral data in the range of 230 ～450nm were analyzed by Euclidean distance and principal component analysis（ PCA）.The results showed that,the RSDs of stability within 10 h,accuracy and repeatability of the method,were0.04% ～1.73%,0～2.87% and 0.03% ～0.63%,respectively.The Euclidean distance value of samples among different origins differed significantly from 0.26 to 6.52. PCA showed that the cumulative contribution rate of the first three factors was 86.848%,which could reflect the most information of the samples,and the two- dimensional projection of the first two principal components could distinguish Boletus tomentipes samples from different regions.Ultraviolet spectrum combined with Euclidean distance and principal component analysis（ PCA） can rapidly identify different origin of Boletus tomentipes.