6-苄氨基腺嘌呤对蕨菜木质化的影响

Effect of 6- benzyl aminopurine on postharvest lignification on Pteridium aquilinum

为了有效地抑制采后蕨菜木质化,延长蕨菜的货架期。分别比较25mg/L 6-苄氨基腺嘌呤（6-benzyl aminopurine,6-BA）及50mg/L 6-BA浸泡处理蕨菜15min后,在13～15℃、相对湿度80%～85%条件下贮藏9d,用清水浸泡作CK,测定苯丙氨酸解氨酶（PAL）、过氧化物酶（POD）、4-香豆酸辅酶A连接酶（4-CL）、肉桂醇脱氢酶（CAD）、过氧化氢酶（CAT）等酶及丙二醛（MDA）含量、木质素含量、蛋白质含量。结果表明：6-BA处理能明显降低与木质素合成相关酶PAL、POD、4-CL、CAD等活力（p〈0.05）;各处理中木质素含量在贮藏期间均呈上升趋势,蛋白质含量呈下降趋势;在贮藏期间,6-BA处理组中木质素及MDA的含量低于CK组;6-BA处理及CK组蛋白组含量均呈下降趋势,但6-BA处理明显抑制蛋白质含量下降和明显提高CAT酶活力。综上50mg/L 6-BA能较好地抑制采后蕨菜的木质化。

In order to effectively restrain post- harvest lignification and extend the shelf life of Pteridium aquilinum,Post- harvest Pteridium aquilinum was soaked in the solution of 25 mg / L and 50 mg / L 6- BA solution for 15 min; then stored under 13～ 15℃,RH 80% ～ 85% for 9d and the control（ CK） was soaked in water and then as same as6- benzyl aminopurine（ 6- BA） treatment. The enzyme activity of phenylalnine ammonialyase（ PAL）,peroxidase（ POD）,4- coumarate Co A ligase（ 4- CL）,Cinnamyl alcohol dehydrogenase（ CAD）,Catalase（ CAT） and malondialdehyde（ MDA） content,lignin content and protein content were determined.Results showed that enzyme activity of PAL,POD,4- CL,CAD treated by 6- BA was significantly lower than CK. Lignin content was ascending and protein content was descending during its storage period. Lignin and MDA content of 6- BA treatment was lower than CK.Protein content and enzyme activity of CAT was significantly higher than the control. In conclusion,the lignification process could be inhibited by 6- BA during storage periods.