藤茶提取物中二氢杨梅素对大鼠急性痛风性关节炎模型的影响

Effect of Dihydromyricetin from Cany Tea on Acute Gouty Arthritis Model Rats

目的研究藤茶提取物中二氢杨梅素对大鼠急性痛风性关节炎模型中血清IL-1β、IL-8、TNF-α的影响,初步探讨其治疗急性痛风性关节炎的作用机制。方法采用踝关节腔内注射尿酸钠建立急性痛风性关节炎大鼠模型,以秋水仙碱为对照,观察藤茶提取物中二氢杨梅素对各组大鼠的踝关节肿胀度及血清TNF-α、IL-1β及IL-8水平的调节作用。结果模型组关节肿胀度、IL-1β、IL-8和TNF-α的水平明显高于空白对照组(P<0.05或P<0.01);中高剂量的二氢杨梅素能明显抑制急性痛风性关节炎大鼠的踝关节肿胀度,降低血清TNF-α、IL-1B及IL-8的水平。结论藤茶提取物中二氢杨梅素对急性痛风性关节炎具有良好的治疗作用,其作用机制与抑制血清IL-1β、IL-8和TNF-α的产生有关。

OBJECTIVE To study the effection of dihydromyricetin from Cany tea on acute gouty arthritis model joint swelling degree and rats serum of IL-1β, IL-8, TNF-α, and to explore the action mechanism of the acute gouty arthritis treatment. METHODS Establish rats model of acute gouty arthritis by using the ankle articular injection of sodium urate, the control group use the colchicine and observe the regulation of dihydromyricetin from Cany tea on rats ankle swelling and serum levels of TNF-α, IL-1β, and IL-8 in each group. RESULTS The degree of joint swelling and IL-1β, IL-8, and TNF-α levels of model group were significantly higher than the normal group（P0.05, P0.01）; High and middle dose of dihydromyricetin from Cany tea could significantly inhibit acute gouty arthritis in rats ankle swelling and reduce serum TNF-α, IL-1βand IL-8 level. CONCLUSION Dihydromyricetin from Cany tea has a good role in the prevention and treatment of acute gouty arthritis, its mechanism of action is the inhibition of serum IL-1β, IL-8 and TNF-α production.