HPLC同时测定玉叶金花清热片中3种有效成分的含量

Determination of Three Effective Components in Yuyejinhua Qingre Tablets by HPLC

目的 建立同时测定玉叶金花清热片中栀子苷、穿心莲内酯和脱水穿心莲内酯含量的HPLC。方法 采用AgilentZORBAX SB-C18色谱柱（4.6 mm×150 mm,5μm）,流动相为甲醇-0.2%磷酸水溶液,梯度洗脱,流速为1.0 mL.min 1,检测波长：238 nm（0~10 min,栀子苷）,225 nm（10~22 min,穿心莲内酯）,254 nm（22~35 min）。结果 栀子苷在0.166~2.981μg内线性关系良好（r=0.999 9）,平均回收率为99.4%（RSD=1.2%）;穿心莲内酯在0.146~2.621μg内线性关系良好（r=0.999 9,平均回收率为99.2%（RSD=1.3%）;脱水穿心莲内酯在0.222~3.992μg内性关系良好（r=0.999 7）,平均回收率为100.1%（RSD=1.4%）。结论 该方法简便、快捷、准确,可用于该制剂的质量控制。

OBJECTIVE To simultaneously determine geniposide, andrographolide and dehydroandrographolide in Yuyejinhua Qingre tablets by HPLC. METHODS Agilent ZORBAX SB-C18（4.6 mm×150 mm, 5 μm） column was used. The mobile phase was methanol-water containing 0.2% phosphoric acid with gradient elution mode at the flow rate of 1.0 m L·min-1; the detection wavelength was 238 nm（0-10 min） for geniposide, 225 nm（10-22 min） for andrographolide and 254 nm（22-35 min） for dehydroandrographolide. RESULTS Geniposide had a good linearity in the range of 0.166-2.981 μg, the average recovery was 99.4% with RSD of 1.2%. Andrographolide had a good linearity in the range of 0.146-2.621 μg, the average recovery was 99.2% with RSD of 1.3%. Dehydroandrographolide had a good linearity in the range of 0.222-3.992 μg, the average recovery was 100.1% with RSD of 1.4%. CONCLUSION The method is simple, accurate and reproducible, and can be used for quality control of Yuyejinhua Qingre tablets.