两种抗ENA抗体检测方法的结果观察

An Observation on the Result of Detection Carried out with 2 Anti-ENA Antibody Detection Methods

目的:观察免疫斑点法和免疫印迹法测定抗ENA抗体结果的差异性。方法:选取美国GenBio公司生产的自身抗体检测试剂盒(免疫斑点法)和广州万孚公司生产ENA试剂盒(免疫印迹法),同时检测433例样本中的抗ENA抗体,结果采用McNemar检验,比较这两种方法的检测性能。结果:(1)成组χ2检验:免疫斑点法和免疫印迹法的检测结果有相关性(P=0.000);(2)McNemar检验:免疫斑点法和免疫印迹法检测结果之间有差异(P=0.000);(3)Kappa检验:免疫斑点法和免疫印迹法检测结果的一致性有统计学意义,但一致性程度一般(Kappa=0.664,P=0.000)。结论:在临床检测抗ENA抗体时,对于弱阳性结果、不典型的结果最好用另一种方法来检测,再结合其它指标来综合判断,才能准确地发出检验报告。

Objective：To observe the difference between the anti-ENA antibody detection result obtained through immunospot assay and immunoblot assay.Methods：Test anti-ENA antibody contained 433 samples with the autoantibody test kit（immunospot assay）yielded by American GenBio company and ENA test kit（immunoblot assay）yielded by Guangzhou Wondfo Biotech Co.,Ltd,check the result with McNemar,compare the detection performance of the 2methods.Results：（1）Chi-square test：The result of immunospot assay was correlative to that of immunoblot assay（P=0.000）;（2）McNemar test：The result of immunospot assay was different from that of immunoblot assay（P=0.000）;（3）Kappa test：the consistency between the result of immunospot assay and immunoblot assay was statistically significant,but the consistency degree was around the average（Kappa=0.664,P=0.000）.Conclusion：When testing the anti-ENA antibody clinically,in allusion to the weakly positive results and atypical results,it is better to test with some other methods and combine other indexes to make comprehensive judgments,so as to give out the exact test report.