八门湾红树林土壤小单孢菌的分离及其多样性

Isolation of Micromonosporaceae from Mangrove Soil in Bamen Bay & Its Diversity

采用平板稀释法从海南八门湾红树林潮间带、木果榄和海莲红树根际土壤样品中分离和筛选小单孢菌科放线菌,对其分离方法进行了评价和比较,并通过16S rRNA基因测序探索了八门湾红树林小单孢菌科放线菌的多样性。采用4种预处理方法和5种分离培养基,经过排除重复菌株后共得到115株放线菌。16S rRNA基因测序结果显示,这些放线菌分布在5个科、9个属。其中小单孢菌科的菌株约占全部菌株的90%（105株）,主要分布于3个属：Micromonospora（85%）,Jishengella（9.5%）和Verrucosispora（5.5%）,与其亲缘关系最近的菌株的相似性分布于98.5%~100%之间。

Micromonosporaceae actinomycetes were isolated and screened from soil samples collected from rhizospheric soil of Xylocarpus mekongensis and Bruguiera sexangula in Wenchang Bamen Bay of Hainan intertidal zone adopting dilution-plate method,and the isolation methods were valuated and compared,and the diversity of Micromonosporaceae actinomycetes in Bamen Bay mangrove forest were investigated with 16 S rRNA gene sequencing. Four pre-treatment approaches and five isolation media were used,a total of 115 actinomycetes strains were obtained after removing the repetitive strains. The 16 S rRNA gene sequencing results showed that these 115 actinomycetes belonged to 5 families and 9 genera. Among them Micromonosporaceae strains accounted for 90% of the total（ 105 strains）,and mainly distributed in three genera： Micromonospora（ 85%）,Jishengella（ 9. 5%）,and Verrucosispora（ 5. 5%）,and the similarities of the strain with the nearest osculation distributed from 98. 5% to 100%.