儿童表皮兜甲蛋白及内披蛋白的表达与特应性皮炎的发病机制探讨

The expression of epidermal terminal differentiation protein of children with atopic dermatitis

目的探讨特应性皮炎(atopic dermatitis,AD)儿童急性病损期皮肤2种终末分化蛋白的表达情况,从皮肤屏障功能障碍方面研究AD的发病机制。方法选取中、重度AD患儿作为AD组,通过皮肤环钻术获取皮肤标本,以正常皮肤为对照,对兜甲蛋白(loricrin,LOR)及内披蛋白(involucrin,INV)2种表皮终末分化蛋白进行免疫组化研究。结果发现在AD患儿急性病损期皮肤,2种分化蛋白染色强度较正常对照组均减弱,应用图像分析系统对2种分化蛋白染色结果进行分析显示,与对照组相比,平均光密度、积分光密度明显降低(P<0.05),阳性面积百分比差异无统计学意义(P>0.05)。结论 2种终末分化蛋白表达异常导致的皮肤屏障功能障碍可能是AD发病原因之一。

Ojective To st udy the expression ofth ree ter minal differentiation protein in acute lesions ofatopic der matitis（A D）child ren i n order to explore the mechan ism ofA D. Methods P u nch biopsies were collected from acute lesions ofA D（moderate or severe）child ren and nor mal healthy skin. T he expression offilagg r i n（FLG）,involucr in（I N V） and lor icr in（LOR）were investigated by im munohistochemistry. Results The staining intensit y oft h ree protei n decrea sed i n acute lesional sk i n ofA D subject s wa s fou nd. Mea n opt ical den sit y（m OD, i nteg r al optical density（i OD）sig nificantly decreased in involved A D skin as compared to skin with nor mal subjects（P〈0.05）. Conclusion The abnor mal expression oft h ree ter m i nal different iat ion proteini nduced skin barrier dysfu nct ion, which may play an impor tant role in the pathogenesis ofA D.