交联透明质酸钠细胞支架材料中交联剂残留量的测定

Determination of Crosslinking Agent Residual in Cross-linked Sodium Hyaluronate Cell Scaffold

建立了气相色谱法测定交联透明质酸钠细胞支架材料中交联剂1,4-丁二醇二缩水甘油醚残留量的方法。选用安捷伦HP-5毛细管柱（30 m×0.32 mm,0.25μm）,氢火焰离子化检测器（FID）,载气为氮气,流速为2.0 ml·min-1,柱温采用程序升温,初始温度为90℃,以15℃·min-1升温至250℃,保持8 min;采用外标法定量。1,4-丁二醇二缩水甘油醚浓度在0.133~1.865μg·ml-1的范围内,峰面积和浓度呈现良好的线性关系,相关系数为0.9993;回收率为96.33%~100.57%,相对标准偏差（RSD）为1.40%（n=9）。方法专属性强、重现性好、灵敏度高,可用于交联透明质酸钠支架材料中1,4-丁二醇二缩水甘油醚残留量的测定。

A method for determination of residual 1,4- butanediol diglycidyl ether（ BDDE） in cross- linked sodium hyaluronate cell scaffold by GC chromatography was established. The method was programmed with Agilent HP- 5 capillary column（ 30 m × 0. 32 mm,0. 25 μm） and FID detector. The carrier gas was nitrogen and the flow rate was 2. 0 ml·min- 1. The oven introduced sequential increasing of temperature programming. The initial column temperature was 90 ℃,then raised it to 250 ℃ at a rate of 15 ℃ ·min- 1,and maintained it for 8 min. The BDDE was quantified external standard. The calibration showed a good calibration linearity with the range of 0. 133 ~ 1. 865 μg·ml- 1for BDDE and the correlation coefficient was 0. 9993. The recoveries were in the range of 96. 33 %~ 100. 57 %. The relative standard deviations（ RSD） was 1. 40 %（ n = 9）. The method has good specificity,repeatability and high sensitivity. It can be used for determining the content of residual BDDE in cross- linked sodium hyaluronate scaffold.