2010～2013年广西规模化猪场伪狂犬病毒野毒株感染血清学调查

Serology epidemiology of pseudorabies yield virus from pig farms in Guangxi from 2010 to 2013

【目的】全面了解广西规模化猪场伪狂犬病毒（PRV）野毒株的流行情况,为指导猪场防控和净化伪狂犬病（PR）提供参考依据。【方法】2010-2013年采集广西地区300个规模化猪场的3950份猪血清样品,采用酶联免疫吸附试验（ELISA）进行PRV抗体检测,并对不同地区、种类猪群的PRV感染情况进行分析。【结果】在2010-2013年抽检的300个规模猪场中有23.00%为PRV阳性猪场,3950份猪血清样本中有5.16%为阳性样本,且二者均呈逐年上升趋势。PRV阳性猪场率高于广西平均水平的5个地级市分别为北海（75.00%）、百色（42.86%）、崇左（35.71%）、南宁（25.17%）和柳州（25.00%）,PRV样本阳性率高于广西平均水平的4个市分别是崇左（9.71%）、百色（9.09%）、南宁（6.50%）和钦州（6.12%）。从不同地区来看,感染猪场以桂西和桂南地区较高,PRV阳性猪场率分别为30.00%和27.23%,PRV样本阳性率分别为5.21%和6.80%。种猪的PRV感染率（6.45%）明显高于肉猪（2.52%）。【结论】广西猪群已普遍存在PRV野毒株感染,感染率逐年上升,且以种猪感染风险较高,实际生产中可采取实时野毒抗体监测、淘汰PRV阳性种猪、强化疫苗免疫等一系列综合措施进行防控。

[Objective]Serology epidemiology of pseduorabies virus（PRV） from pig farms in Guangxi was studied to provide theoretical references for control and decontamination of PRV in pig farm. 【Method】During 2010-2013, 3950 sera samples of pigs from 300 pig farms were used to examine the antibody of PRV gp I by ELISA kit. The prevalent situation of PRV of varied types of pig was analyzed in different regions. 【Result 】The results showed that the infection rate of pig farm in 300 pig farms was 23.00%. The positive rate of sample in 3950 sera sample was 5.16%. These data showed an increase trend year by year. There were five cities whose infection rate of pig farm was higher than the average level. They were Beihai（75.00%）, Baise（42.86%）, Chongzuo（35.71%）, Nanning（25.17%） and Liuzhou（25.00%）.There were four cities whose positive rate of sample was higher than the average level. They were Chongzuo（9.71%）, Baise（ 9. 09 %）, Nanning（6.50%） and Qinzhou（6.12%）. The higher infection rate of pig farm and positive rate of sample were recorded in western and southern region of Guangxi, where the infection rate of pig farm was30.00% and 27.23%, respectively, and positive rate of sample was 5.21% and 6.80%, respectively. The infection rate of breeder hogs（6.45%） was higher than that of the meat pig（2.52%）. 【Conclusion】Swine PRV infection in Guangxi is widespread, and both positive rate of pig farm and positive rate of sample show an increase trend year by year.Breeder hogs are more vulnerable to be infected. Therefore, regular monitoring of PRV, eradication of infected breeder pigs and immunization should be taken into account in swine industry.