七氟醚对老龄大鼠空间学习和记忆能力的影响

Effects of sevoflurane on spatial learning and memory ability in senile rats

目的通过Morris水迷宫实验,探讨七氟醚对老龄大鼠空间学习和记忆能力的影响。方法选取18月龄,450~500 g,健康雄性SD大鼠24只,随机分为4组。大鼠于麻醉前进行Morris水迷宫适应性训练3 d,于麻醉结束后1、3、7 d进行M orris水迷宫实验测定空间学习和记忆能力。结果 1定位航行实验。组间比较：麻醉后1、3 d,I组、S1组逃避潜伏期较C组延长（P〈0.05）;麻醉后3 d,I组、S1组总游泳距离较C组延长（P〈0.05）;麻醉后7 d,I组逃避潜伏期和总游泳距离较C组、S1组延长（P〈0.05）。组内比较：与麻醉后1 d比较,麻醉后3、7 d各组逃避潜伏期和总游泳距离均延长（P〈0.05,P〈0.01）。2空间探索实验。S1组、S2组的平台象限的停留时间（TP）和TP与总游泳时间的百分比（TP/T）与C组比较差异均有统计学意义（P〈0.05）,I组与C组比较差异有统计学意义（P〈0.01）。结论低浓度七氟醚对老龄大鼠早期空间学习和记忆能力无明显影响。高浓度七氟醚、异氟醚均可引起老龄大鼠早期空间学习和记忆能力一过性下降,但这些变化均在麻醉后第7天趋于恢复,且七氟醚恢复较异氟醚快。

Objective To investigate the effects of sevoflurane on spatial learning and memory ability in senile rats via M orris water maze experiment. Methods Tw enty-four healthy male SD rats aged 18 months weighing 450 ~500 g were randomly divided into four groups. The spatial learning and memory ability of rats performed adaptive training of M orris water maze for three days before anesthesia were subsequently assessed with M orris water maze at 1,3and 7 d after anesthesia. Results 1The navigation test. Compared with group C（ 1,3 d after anesthesia）,the escape latency of both group I and group S1 were longer（ P〈0. 05）; The total sw imming distance of group I and group S1 were longer at 3 d after anesthesia（ P〈0. 05）. Compared with group C and group S1,both the escape latency and the total swimming distance of group I were extended（ P〈0. 05） at 7 d after anesthesia. The escape latency and the total sw imming distance of all groups were more extended at 3 d（ P〈0. 05） and 7 d（ P〈0. 01） after anesthesia compared with the above indexes at 1 d after anesthesia. 2The spatial probe test. There were significant differences in the residence time in platform quadrant（ TP）,the percentage of TP and the total sw imming time（ TP / T） between group S1/group S2 and group C（ P〈0. 05）,and there were significant differences between group I and group C（ P〈0. 01）.Conclusion Sevoflurane with low concentration has no obvious effect on early stage of spatial learning and memory ability in senile rats. Both sevoflurane and isoflurane with high concentration can cause transient decline on early stage of spatial learning and memory ability in senile rats,how ever,the changes tend to restore from the 7th day after anesthesia,moreover,sevoflurane restores faster than isoflurane.