消瘤平体内外对人宫颈癌HeLa细胞生长及凋亡相关蛋白的影响

Effect of Xiao liuping on human cervical carcinoma He La cells growth and apoptosis-related proteins in vivo and in vitro

目的研究消瘤平体内外对人宫颈癌He La细胞生长的影响,以及对凋亡相关蛋白Bcl-2、Bax、Fas和Survivin含量的影响。方法常规培养He La细胞,加入不同浓度的消瘤平,M TT法分别检测培养3个时段后对细胞生长的影响。He La细胞接种于BALB/C-nu裸鼠,随机分为5组,阴性对照组、5-fu阳性对照组以及消瘤平3个剂量组,另设空白对照组,观察荷瘤裸鼠的一般状况。腹腔注射给药4周后,取瘤块和脾脏,称重计算抑瘤率和脾脏指数。取血,免疫组化法检测凋亡相关基因Bcl-2、Bax、Fas、Survivin蛋白的含量。结果消瘤平体内外均能抑制He La细胞的生长;体内可明显增加荷瘤裸鼠的脾脏指数;中、高剂量的消瘤平可促进Bax的表达,抑制Bcl-2和Survivin的表达,高剂量的消瘤平还促进Fas的表达。结论消瘤平抗宫颈癌的作用机制与抑制He La细胞增殖,以及Bcl-2、Bax、Fas和Survivin基因蛋白诱导的凋亡有关。

Objective To investigate the effect of Xiao liuping on growth and the apoptosis-related protein Bcl-2,Bax,Fas and survivin content of human cervical carcinoma He La cells. Methods Cells were cultivated in vitro,He La cells in culture medium in vitro were given with different concentrations of Xiao liuping,the inhibitory rate of the cells was measured by M TT assay. He La cells were seeded in BALB / C-nu mice,the tumor-bearing nude mice were divided into 5 groups randomly ： control group,5-Fu group,Xiao liuping three doses groups,and the blank control group was set,the general condition of nude mice was observed. After 4 weeks,the tumor and spleen weight was determined,the tumor-inhibition rate and spleen index were calculated. The expression of apoptosis-related proteins Bcl-2,Bax Fas and Survivin in blood were determined by immunohistochemistry. Results Xiao liuping inhibited the proliferation of He La cells in vitro and in vivo,increased the spleen index of nude mice significantly,the medium and high doses of Xiao liuping promoted the expression of Bax,and inhibited the expression of Bcl-2 and Survivin,high dose of Xiao liuping promoted the expression of Fas. Conclusion The anti-tumor mechanism of Xiao liuping related to the inhibition of the proliferation,as well as Bcl-2,Bax,Fas and Survivin induced apoptosis.